Optimization of Xylanase Secretion from Paenibacillus macquariensis

Order of Publishing in Issue: 
6
Volume :6
Issue :2
April, 2012
Page No: 
190-195
Authors: 
Meeta Sharma and Anil Kumar*
Address: 
School of Biotechnology, Devi Ahilya University, Khandwa Rd., Indore-452001, India
Email-ID: 
ak_sbt@yahoo.com

Abstract
A sporulating bacteria having potential to produce xylanase has been isolated from the soil mixed with petroleum products. The isolated bacteria retained violet color upon gram straining revealing its Gram positive nature. This strain was identified as Paenibacillus macquariensis based on biochemical characterization and deposited in MTCC with an accession number RC1819. The bacteria showed growth at wide variability of pH ranging from pH 5.2 to 10.0 and the temperature ranging from 25 to 37oC. The optimum pH for xylanase production has been found to be in the range of pH 8.2 to 8.6 and the optimum temperature for xylanase production is found to be 37°C. It secreted xylanase in the culture medium (extra-cellular xylanase) in the presence of 2% xylan. It secreted nearly 4.50 units of xylanase per ml of culture medium. The data revealed that Paenibacillus macquariensis has high potential for xylanase secretion and can be used for industrial applications.

Keywords: 
Nil
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