Optimization of tannase production from Aspergillus foetidus using statistical design methods

Order of Publishing in Issue: 
6
Volume :2
Issue :4
October, 2008
Page No: 
523 -530
Authors: 
R.B.Naidu, N.Saisubramanian, S.Sivasubramanian, D.Selvakumar, S.Janardhanan and R. Puvanakrishnan*
Address: 
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai-600 020

Tannase, an industrially important enzyme,  catalyzes the hydrolysis of gallic acid esters andhydrolysable tannins to gallic acid. In this study, the production of tannase from Aspergillusfoetidus MTCC 6322 by submerged culture fermentation was undertaken. Statistical designmethods such as Plackett-Burman and a factorial central composite design (CCD) were employedfor optimization. Two fold increase in tannase activity (30 units/ml/min) was observed incomparison to the medium optimized by the conventional one factor at a time method (15units/ml/min). In addition, the number of nutrient factors for the production of tannase weresignificantly reduced from ten to four after optimization.

Keywords: 
Aspergillus foetidus; tannase; submerged fermentation.
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