Optimization of Protocols for Callus Induction, Regeneration and Acclimatization of Sugarcane (Saccharum officinarum L.) Cultivar CO-86032

Order of Publishing in Issue: 
5
Volume :7
Issue :4
Regular
October, 2013
Page No: 
862-869
Authors: 
Srinath Rao and FTZ Jabeen
Address: 
Plant Tissue Culture and Genetic Engineering Lab Department of Post-Graduate Research and Studies in Botany, Gulbarga University, Gulbarga 585 106, Karnataka, India

An efficient protocol for induction of callus and regeneration of a high yielding sugar cane var CO-86032 has been developed and reported here. Callus induction from leaf sheath explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s(MS) medium supplemented with different auxins viz, 2,4-D, 2,4,5-T, NAA, dicamba and picloram (1-3 mgl-1). Among different auxins, 2,4-D at 1mgl-1 supplemented with 2% sucrose + 300mgl-1 PVP was found favorable in inducing callus and preventing browning. Addition of coconut milk and Kn further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg1-1Kn (1994±0.39mg). Calli were further evaluated for regeneration. MS medium supplemented with 2mg/l Kn+1mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass(168±0.54). Highest number of root emergence (38±0.21) and maximum root length (6.8±0.64cm) was achieved on MS medium supplemented with 5mgl-1 NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand and black soil (1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.

Keywords: 
Saccharum, callus, leaf explants, organogenesis, sugarcane.
AttachmentSize
Paper-5.pdf913.86 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login