Optimization of process parameters for Poly Hydroxy Butyrate Production from Isolated Acinetobacter nosocomialis RR20 through Submerged Fermentation

Order of Publishing in Issue: 
5
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
159-168
Authors: 
A. Ranganadha Reddy[a], T.C. Venkateswarulu[a]*, P. Sudhakar[c], S. Krupanidhi[a], K. Vidya Prabhakar[b]*
Address: 
[a & a*]Department of Biotechnology Vignan’s Foundation for Science, Technology & Research, Valdamudi-522 213, India
Address: 
[b]*Department of Biotechnology Vikrama Simhapuri University SPSR Nellore-524003, India
Address: 
[c]Department of Biotechnology Acharya Nagarjuna University Guntur-522510, India

Poly Hydroxy Butyrate member of polyhydroxyalkanoates family and is generally used as an alternative to polypropylene based plastic. Production of Poly Hydroxy Butyrate, biodegradable polymer from industrial wastes has several advantages such as recycle of waste and the production of high valuable products. It has been isolated from various sources till date. In this study, different strains of isolated bacteria were evaluated for their PHB productivity, but Acintobacter nosocomialis RR20 strain resulted highest production. Therefore, optimization of process parameters was determined in batch fermentation mode using one-parameter -at atime approach for enhanced production of PHB. The influence of physical and chemical variables namely incubation temperature, incubation time, inoculums size, pH, carbon source, nitrogen source and mineral salts were studied for improving the production of PHB. Maximum PHB was found 4.17 g/L at optimized conditions of incubation period 48 h, temperature 37oC, pH 7.0, inoculums size of 4%, 30g/L molasses, 3g/L Ammonium sulphate and MgSO4, 0.3g/L.

Keywords: 
PHB, A.nosocomialis RR20, Shake flask culture, Process variables.
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