Optimization of Process Parameters for High Yield Production of Exo-Inulinase from Trichoderma asperellum RSBR08 by Using Solid State Fermentation

Order of Publishing in Issue: 
Volume :14
Issue :4
October, 2020 - December, 2020
Page No: 
Babul Reddy G[1], Karuganti Sukumar[2], G. Swarnabala G [1]*
[1]R & D Center of SOM Phytopharma (India) Limited, Hyderabad, India, 502325
[2]Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Palkalaiperur, Tiruchirappalli 620024, Tamil Nadu, India.

Inulinase is an industrially applicable enzyme which is used widely in the production of ultra high fructose syrups and fructooligosaccharides. Production of inulinase enzyme in a cost effective manner is the major challenge faced. In the present research study, Trichoderma asperellum RSBR08 which has the capability to produce high exo-inulinase enzyme is produced by using the solid state fermentation. Process parameters for production of exo-inulinase by using solid state fermentation were optimized. Results showed high exo-inulinase production in garlic as substrate (199.2±6.3 U/gds), 45% moisture percentage (228.4±3.4), 26oC temperature (219.1±5.8) and 5.0 pH (213.6±4.5). Effect of different metals viz. Mg2+,Zn2+, K+,Ca2+, Na+,Mn2+ and Hg2+ were studied on the production of exo-inulinase enzyme in which Ca2+ induced the enzyme production (196.3±5.6) whereas Hg2+ has inhibited the inulinase production (37.0±3.3). Based on the above results, the solid substrate fermentation parameters optimized can be used industrially.

Exo-inulinase, Metal ions, Solid state fermentation, Trichoderma asperellum
PDF487.66 KB

To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.



Upcoming 0 events

  • No upcoming events available

User login