Optimization of culture conditions of a novel glucan producing glucansucrase from Leuconostoc dextranicum NRRL B-1146

Order of Publishing in Issue: 
3
Volume :2
Issue :2
March, 2008
Page No: 
40-48
Authors: 
Avishek Majumder and Arun Goyal*
Address: 
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India

The effect of nutrients on the production of glucansucrase from Leuconostoc dextranicumNRRL B-1146 was studied using one variable at a time approach. The culture conditions forglucansucrase production were also optimized. The maximum enzyme production was obtainedat an optimum temperature of 28°C and under static culture condition. The increase in sucroseconcentration from 2% (control) to 4% in the culture medium gave an increase ofglucansucrase activity by 25%. Yeast extract did not affect the enzyme production. However, othernitrogen sources like beef extract and peptone resulted in 10% and 16% increase inglucansucrase activity, respectively. On increasing the K2HPO4 from 2 to 2.5% theenzyme activity in the culture supernatant increased by 25%. There was a significantincrease in the glucansucrase activity by addition of Tween 80 as the production was enhanced by65% by 1% of Tween 80. An increase in MnSO4 concentration to 0.01% caused 37% increase inthe enzyme production. The salts like NaCl, CaCl2 and FeSO4 also enhanced the enzymeproduction significantly and showed increase of 17, 26 and 20%, the enzyme productioncorrespondingly with 0.005% NaCl, 0.002% CaCl2 and 0.005% FeSO4.

Keywords: 
glucansucrase; Leuconostoc dextranicum; glucan
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