Optimization and Production of Fibrinolytic Protease (GD kinase) from Different Agro Industrial Wastes in Solid State Fermentation

Order of Publishing in Issue: 
7
Volume :7
Issue :3
July, 2013
Page No: 
763-772
Authors: 
E. Venkata Naga Raju[1] and G. Divakar [2]
Address: 
[1] Department of Biotechnology, Acharya Nagarjuna University, Nagarjunanagar – 522 510, Guntur – 522 510, A.P., India
Address: 
[2] Department of Biotechnology, Acharya & B.M. Reddy College of Pharmacy, Soladevanahalli, Hesaraghatta, Bangalore – 560 090, India

Abstract :

The cost of fibrinolytic protease (GD kinase) production may be significantly decreased by using inexpensive carbon substrates like agricultural residues. The optimization of different physical parameters such as various substrates, initial moisture level, pH, substrate particle size, incubation temperature, inoculum size, incubation period and chemical parameters such as additional carbon and nitrogen sources were studied for the production of fibrinolytic protease (GD kinase) in solid-state fermentation using mutant Bacillus cereus GD55. The maximum fibrinolytic protease (GD kinase) production was observed with apple pomace (52.20 ± 0.21 U/g), peptone (50.87 ± 0.21 U/g), NH4NO3 (50.19 ± 0.65 U/g), incubation period 4 Days (48.32 ± 0.24 U/g), inoculum size 2.0% (46.24 ± 0.98 U/g), incubation temperature 35°C (40.85 ± 0.19 U/g), substrate particle size 1.2 mm (40.15 ± 0.17 U/ g), initial pH 8 (38.39 ± 0.36 U/g), initial moisture level 70% (34.28 ± 0.10U/g), carbon source fructose (46.64 ± 0.13 U/g) and least fibrinolytic protease (GD kinase) production was observed with rice chaff (12.8± 0.12 U/g) in the production medium.

Keywords: 
Solid-state fermentation, Bacillus cereus GD55, Optimization, fibrinolytic protease (GD kinase).
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