Optimization and Production of extracellular amylase from newly isolated Aspergillus species by Submerged Fermentation

Order of Publishing in Issue: 
8
Volume :4
Issue :3
July, 2010
Page No: 
795-800
Authors: 
Murali Krishna Chimata[1], Sasidhar Pasumarthi[1] and Suresh Challa[2]*
Address: 
[1] Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
Address: 
[2] Department of Biochemistry, National Institute of Nutrition, Tarnaka, Hyderabad - 500 007, India

Abstract

The production of extracellular amylases by SmF was investigated employing our laboratory isolate Aspergillus sp.MK07. Various process variables namely Incubation Period, Temperature, pH, Inoculum level were tested. Varying concentrations of CSL from 1 to 4% were supplemented to the synthetic media used in the study to evaluate the nitrogen source effect on the media with our laboratory isolate Aspergillus sp.MK07. Corn steep liquor played an important role in amylase production and an additional supplementation of 2% CSL to the media increased amylase production compared to control without CSL in the media. Among the different parameters tested pH, Temperature and substrates played a very important role. Highest amylase production was obtained with a temperature of 30oC, pH 5.0, Inoculum level 5%, incubation period of 72 h and among the carbon substrates tested highest amylase production was obtained when Sucrose was used as a substrate. Highest amylase production obtained under all optimized parameters was 91 U/ml.

Keywords: 
Submerged, Optimization, Aspergillus, Fermentation, Amylases
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