Optimal production conditions, extraction, partial purification and characterization of inhibitory compound(s) produced by Streptomyces Ds-104 isolate against multi-drug resistant Candida albicans

Order of Publishing in Issue: 
5
Volume :2
Issue :2
July, 2008
Page No: 
402 -420
Authors: 
Ismail Saadoun* and Asia Muhana
Address: 
Department of Applied Biological Sciences, Jordan University of Science and Technology Irbid-22110, Jordan
Email-ID: 
isaadoun@just.edu.jo

A Streptomyces Ds-104 isolate was found to inhibit four multi-drug resistant Candidaalbicans pathogens with an optimal production of the inhibitory compound(s) in starch, casein,nitrate broth (SCNB) and inhibition zone diameters ranging between 14 and 18 mm. Thesewere significantly different (P= 0.05) from what was obtained in nutrient, oatmeal, inorganic saltstarch, and yeast extract, malt extract broth media. When Ds-104 isolate was grown in SCNBsupplemented with glucose, 15 g l-1; KNO3, 2.5 g l-1; NaCl, 10 g l-1; and no KH2PO4, and incubatedat 28°C for 10 days with shaking at 100 rpm, the inhibition zone diameter ranged between 25 and30 mm, increased by 1.5-folds and significantly different (P = 0.05) from the other culturesupplements and conditions. The minimum inhibitory concentration (MIC), U.V absorptionspectrum and TLC analysis of the water soluble of evaporated ethanol crude extract revealed MICof 6.25 mg mL-1, maximum absorbance at 205 nm, and an Rf value of 0.75, respectively. TheRf value and UV absorption spectrum of the active compound(s) in the crude extract aredifferent from the tested drugs (fluconazole and amphotericin B) which may suggest the noveltyof the inhibitory compound(s) produced by the Streptomyces Ds-104 isolate.

Keywords: 
Bioactive compounds, Candida albicans, Inhibitory compounds, Streptomyces
AttachmentSize
Paper 5.pdf176.34 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login