Novel Aqueous solvent based method for protein based nanoparticles

Order of Publishing in Issue: 
13
Volume :2
Issue :4
October, 2008
Page No: 
575 -584
Authors: 
M. M. Ibrahim1,2, O. A. Sammour3, M. A. Hammad2, N. A. Megrab2, X. Li1 and B. Jasti1*
Address: 
1Thomas J. Long school of Pharmacy, University of the Pacific, Stockton, CA; 2Dept. of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt; 3Dept. of Drug Technology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
Email-ID: 
bjasti@pacific.edu

The objective of the present study is to develop a novel method for the preparation ofpoly(D,L-lactide-co-glycolide)(PLGA) nanoparticles for protein/peptide delivery, and compare this method with traditional solvent evaporation methods for nanoparticle particlesize and entrapment efficiency. The nanoparticles were prepared by three different methods namely:w/o/w emulsification-solvent evaporation method (ESE), nanoprecipitation method, andnovel aqueous mixed micelle (MM) method. The prepared nanoparticles were evaluated as drugcarrier systems using bovine serum albumin (BSA) as a model peptide. The physicochemicalcharacteristics of the nanoparticles; morphology, particle size, zeta potential, and the effect ofdifferent parameters (method of preparation, time of stirring, drug concentration, and polymerconcentration) on particle size and protein encapsulation efficiency percent (EE%) werestudied. Severe aggregation of nanoparticles was noticed after freeze drying, especially for MMmethod. To reduce or completely prevent nanoparticle aggregation during freeze drying,three sugar cryoprotectants (glucose, trehalose,and mannitol) were evaluated. Results showedthat all the preparation methods yielded greater than 90% EE of BSA into nanoparticles.Increasing the time of stirring during preparation decreased the particle size and was of a littleeffect on EE% in case of ESE and nanoprecipitation methods. On the other hand,the time of stirring has no effect on the particlesize and EE% in case of MM method. Increasing the drug concentration and PLGA concentration was found to increase both particle size and EE%.Both glucose and trehalose protected nanoparticles from aggregation during freezedrying while mannitol had increased their aggregation. In conclusion, this study suggestedthe potential of preparing PLGA nanoparticles using aqueous solvent that can be used for loadingpeptide drugs.

Keywords: 
Emulsification, Nanoprecipitation, Mixed micelle, Nanoparticles, PLGA
AttachmentSize
575-584.pdf159.06 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login