Intervertebral Disc Proteome Database in Human (TNAUPDB –Human Proteome)

Order of Publishing in Issue: 
10
Volume :8
Issue :4
October, 2014
Page No: 
423-427
Authors: 
Veera Ranjani. R[*1], Sreemol. G[1], Raveendran. M[1], Senthil. N[1], Gnanam. R[1], Rishi. M. K[2] and Rajasekaran, S[2].
Address: 
[1]Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Address: 
[2]Ganga Orthopedic Research and Education Foundation, Coimbatore, Tamil Nadu, India
Email-ID: 
rajaranji@gmail.com

Human intervertebral disc (IVD) proteome is an open accessible database of Tamil Nadu Agricultural University Proteome DataBase (TNAU PDB) that focuses on proteome of human intervertebral disc. Currently, the database contains reference maps of Two- Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of proteins obtained from degenerated intervertebral disc. The initial 2DPAGE and MALDI TOF studies have revealed that abundantly expressed 30 protein spots. The database provides information about experimentally identified properties, such as molecular weight, pI value and protein identities obtained using MALDI-TOF and MASCOT analysis. This database runs on WAMP server with HTML as the front end and MySQL as the backend using PHP as interface and it is hosted in TNAU genomics domain. The basic intention of this database is to provide information about intervertebral disc proteome. Further information on differentially expressed proteins on comparison with healthy and degenerated intervertebral disc tissue will help us to gain insights into the underlying molecular mechanism in disc degenerative disease. This database will be a valuable biological resource for the treatment of disc degenerative disease.

Keywords: 
Intervertebral disc, Disc degenerative disease, database, proteomics, proteome database, TNAUPDB, TNAU genomics.
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