Identification of Soil Enriched Microorganisms using 16S rDNA analysis for Crop Prodctivity

Order of Publishing in Issue: 
Volume :8
Issue :4
October, 2014
Page No: 
Shobit Gupta, Abhishek Singh, Pooja Chaudhary, Mukeshwar Pandey, K M Singh and Surendra K Chikara[*]
Xcelris Genomics, Xcelris Labs Ltd, Opp Satyagrah Chawani, Old Premchandnagr Road, Bodakdev, Ahmedabad, Gujarat, India

In the present study, occurrence of cultivable bacteria in the soils of Chandan plant (Santalum album) and Bamboo plant (Bambussa bambus ) was investigated. Soils are likely to include species of various bacterial phyla, so different colony characteristics isolates were selected and 16S rDNA sequences were amplified from the samples. Five genera (MS2) and 3 genera (MS1) were identified by 16S ribosomal RNA gene sequence analysis (similarities to known 16S rDNA sequences). The majority of bacteria were Bacillus belonged to Firmicutes in MS2. While the presence of Pseudomonas, Micrococcus and Enterobacter were less. The endophytic bacterial community was also present in MS1 sample. In addition, phylogenetic analyses were also used to infer the makeup of bacterial communities in the soil samples. As a result, our isolates (from both soil) nicely clustered with reference sequences (16S rDNA sequences). Our results show that the population diversity of cultivable bacteria is abundant in the both soil types. These cultivable bacterial consortium may be used in crop production.

Bamboo; Chandan 16 s RNA gene; Soil
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