Molecular evolution of Thermophilic Geobacillus species isolated from Tattapani Hotspring (India) and the Worldwide Distribution

Order of Publishing in Issue: 
2
Volume :14
Issue :1
January, 2020 - March, 2020
Page No: 
16-32
Authors: 
Parul Sharma[1], Sonika Gupta[1], Anuradha Sourirajan[1], David J. Baumler[2,3,4], Kamal Dev[1,2],*
Address: 
[1]Faculty of Biotechnology, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India, PIN Code: 173212
Address: 
[2]Department of Food Science and Nutrition, [3]Microbial and Plant Genomic Institute, and [4]Biotechnology Institute, University of Minnesota-Twin Cities St. Paul, Minnesota, USA

Four thermophilic bacterial isolates PW11, PW12, PW13 and PS4 were isolated from Tattapani Hot Spring. The microbial isolates showed optimum growth at 70ºC and at pH 6-7. Both Na+ and K+ ions were detected at 0.11 M and 1.7 mM respectively in the hot spring water. Interestingly, two bacterial isolates PW12 and PW13 showed growth in the presence of 0.5M KCl, whereas PW12 could grow in the presence of 0.75 M KCl and 0.75 M NaCl. All the four thermophilic isolates were rod shaped Grampositive bacteria, and were analyzed by randomly amplified polymorphic DNA (RAPD) using five random primers and produced PCR fragments ranging from 250 – 6000 bp in size. Further, PW11, PW12, PW13 and PS4 were analyzed by PCR amplification of gene encoding 16S rDNA. 16S rDNA sequence analysis by BLAST search identifies PW13 as Geobacillus thermoleovorans, PW11 as Geobacillus kaustrophilus, PS4 and PW12 as Geobacillus toebii with GenBank accession nos. KF751757, KF751758, KF751759, and KJ509869 respectively.

Keywords: 
Thermophiles, RAPD, 16S rDNA sequencing, Phylogenetic relationship, Geobacillus, BLAST
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