Modelling and Optimization of L-Asparaginase Production from Bacillus stratosphericus

Order of Publishing in Issue: 
10
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
390-405
Authors: 
Madhuri Pola[1], Chandrasai Potla Durthi[1], Satish Babu Rajulapati[1]*, Rajeswara Reddy Erva[2]
Address: 
[1]National Institute of Technology, Warangal
Address: 
[2]National Institute of Technology, Andhra Pradesh

L-Asparaginase has gained importance as one of the most important medications used to treat Acute Lymphocytic Leukemia (ALL). The goal of the current study is to maximize the LAsparaginase activity besides minimizing the LGlutaminase activity. Shorter half-life and side effects remain the drawbacks of the commercially available L-Asparaginase drugs. To overcome the drawbacks, L-Asparaginase has been isolated from endophytes of Ocimum tenuiflorum. The novel endophyte shown highest L-Asparaginase activity and it was identified as Bacillus stratosphericus using 16S rRNA sequencing analysis. With unoptimized medium, the maximum activity for L-Asparaginase was 7.81 IU/ml and for LGlutaminase 6.51 IU/ml at 48h, pH-7.0 and temperature 25°C. It was observed that the LAsparaginase activity was increased to 18 IU/ml by (One-Factor-at-a-Time) OFAT method. Further medium was optimized by Plackett-Burman method. It was observed that Time, Temperature, pH and L-Asparagine were identified as significant variables, which were further optimized by Response Surface Methodology (RSM). After RSM, the L-Asparaginase activity increased to 25.28 IU/ml whereas the L-Glutaminase activity diminished to 3.09 IU/ml.

Keywords: 
L-Asparaginase, ALL, endophytes, OFAT, Plackett-Burman method, RSM.
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