Ligninolytic enzyme activities alternate with mushroom production during industrial cultivation of Pleurotus ostreatus on wheatstraw- based substrate

Order of Publishing in Issue: 
1
Volume :2
Issue :4
October, 2008
Page No: 
478 -492
Authors: 
Martin Rühl, Christoph Fischer and Ursula Kües*
Address: 
Molecular Wood Biotechnology and Technical Mycology, Büsgen-Institute, Georg-August-University Göttingen, Büsgenweg 2, 37077 Göttingen, Germany
Email-ID: 
ukuees@gwdg.de

Two commercial Pleurotus ostreatus strains (030 and K12) were cultivated underindustrial conditions on wheat straw blocks 17-18 kg in weight. Within 10 weeks of cultivation,three flushes of fruiting body production were obtained with total yields of 4.2 and 4.1 kgmushrooms/substrate block and a biological efficiency (BE = total yield of fresh mushroomsper dry weight of substrate) of about 95%, respectively. Ligninolytic activities were followedup within the substrate over the cultivation periods. Laccase, manganese peroxidase (MnP) andversatile (manganese-independent) peroxidase (VP) activities were high in vegetative growthphases within the substrate and dropped upon initiation of fruiting body formation to be hardlydetectable at the harvest of the mature mushrooms. Directly after harvest at all observedflushes, there was a sharp increase in all three enzymatic activities in the substrate for bothstrains (maximum activities for laccase 1.1-1.3 U/ml, for MnP 15-16 U/ml and for VP1.1-1.4 U/ml). Enzymes can be extracted by pressing the spent mushroom substrates (SMS).Potential applications for the press juice or the enzymes in the SMS are discussed.

Keywords: 
Laccase, peroxidase, fruiting bodies, spent mushroom substrate (SMS), solid state fermentation (SSF)
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