Isolation and Characterization of Streptomyces sp. from Durg District of Chhattisgarh for Antimicrobial activity

Order of Publishing in Issue: 
10
Volume :5
Issue :1
January, 2011
Page No: 
1043-1053
Authors: 
N. Singh* and V. Rai
Address: 
Microbiology and Biochemistry Lab, School of Studies in Life Sciences Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, India
Email-ID: 
nevi0007@gmail.com

The present work was carried out in order to establish a suitable protocol for screening program for antimicrobial producing Streptomyces sp. from soil sample of Durg District of Chhattisgarh, INDIA. Fifty seven Streptomyces isolates with distinct characteristics were isolated and purified by serial dilution method on starch casein nitrate agar medium. These isolates were screened for their antimicrobial activity against twenty one test organisms obtained from the Microbial Type Culture Collection and Gene Bank (MTCC), IMTECH, Chandigarh. Morphological, physiological and biochemical characteristics indicated that these isolates belong to the genus Streptomyces and characterized as Streptomyces rimosus,Streptomyces chromogenus, Streptomyces rouchi-3, Streptomyces antibioticus-3, Streptomyces filipinensis, Streptomyces atroolivaceus, Streptomyces exfloliatus-2, Streptomyces halstedii, Streptomyces cyaneus, Streptomyces violaceus, Streptomyces oliovaceoviridis, Streptomyces lydicus, Streptomyces phaeochromogenes, Streptomyces chromofuscus . Among fifty seven isolates, nineteen exhibited antimicrobial activity against test pathogens, and out of these nineteen isolates, 11 isolates exhibited both antibacterial and antifungal activity while 8 exhibited only antibacterial activity.

Keywords: 
Streptomyces sp., Antimicrobial activity, Screening, Characterization
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