Influence of Sucrose and Plant Growth Regulators on Growth and Forskolin Production in Callus cultures of Coleus forskohlii

Order of Publishing in Issue: 
1
Volume :10
Issue :1
January, 2016
Page No: 
46-55
Authors: 
G. Swaroopa, M. Anuraadha and T. Pullaiah
Address: 
Department of Botany, Sri Krishnadevraya University, Anantapur 515003, A.P., India

An efficient protocol for callus culture, suspesion culture and forskolin production in Coleus forskohlii has been described. Best explant for callus induction was leaf and best medium was MS medium. Of all the concentrations tried sucrose at 3% and 4% resulted in higher biomass accumulation. The maximum callus biomass production was observed on 2, 4-D supplemented medium at 2mg/l concentration when compared to other auxins and cytokinins. Of all combinations 3mg/ l NAA + 1mg/l BAP resulted in highest percentage of callus induction and biomass production. This combination in the medium resulted in high percentage of forskolin, but less biomass compared to 2 mg/l 2, 4-D. Maximum production of forskolin was observed on the 14th day of callus culture while in suspension culture maximum production of forskolin was observed on the 10th day of culture

Keywords: 
Forskolin, Coleus forskohlii, callus cultures, suspension cultures, secondary metabolites.

 
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