Impact of Nutritional factors verses Biomass and Serralysin Production in isolated Serratia marcescens

Order of Publishing in Issue: 
8
Volume :6
Issue :4
October, 2012
Page No: 
449-457
Authors: 
P. Lakshmi Bhargavi, B. Sudheer Kumar and R. S. Prakasham
Address: 
Bioengineering and Environmental Centre Indian Institute of Chemical Technology, Hyderabad – 500 607, India
Email-ID: 
prakasam@iict.res.in

Abstract

A high proteolytic enzyme producing marine isolate has been evaluated for its extracellular protease production with respect to different major nutritional sources. The phylogenetic analysis (16S rRNA ribotyping) of the isolated strain revealed that the strain belongs to Serratia marcescens and the sequence has been submitted to EMBL under the accession No. HE613732. Carbon source utilization profile indicated that the isolate has potential to use wide range of carbohydrates ranging from monosaccharides to polysaccharides. This isolate produces cellulase, amylase and chitinase extracellularly. Among tested carbon sources, dextrose among monosacchardies, maltose among disaccharides and starch among polysaccharides supported the best growth as well as enzyme production. Maltose among all carbon sources revealed high ratio of biomass growth Vs enzyme yield. Type of complex nitrogen source influenced the enzyme and biomass production independently. Tryptone supported more than 95% improved enzyme yields compared to combination of yeast extract and peptone. Present study revealed that growth and enzyme production in this isolate is differently regulated by type of complex nitrogen source. Yeast extract supplementation supported for maximum biomass development while other complex nitrogen sources supported equally for biomass and enzyme production. To the best of our knowledge, this strain revealed higher enzyme yields compared to literature reports suggesting commercial potential of this isolate.

Keywords: 
Enzyme, Fermentation, Isolation, Protease, Serratia marcescens.
AttachmentSize
Paper-13.pdf55.57 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login