Immunological and Antioxidant Response of Litopenaeus vannamei fed With Lactobacillus species under WSSV challenge

Order of Publishing in Issue: 
5
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
43-52
Authors: 
Gujjula Mary Sandeepa[1]* and K. Ammani[2]
Address: 
[1]Department of Biotechnology, Vikrama Simhapuri University, Nellore – 524001, India
Address: 
[2]Department of Microbiology, Acharya Nagarjuna University, Guntur – 522 510, India

Two-phased experiment was conducted to investigate the effects of dietary supplementation of probiotic bacterium on shrimp physiology. In first phase shrimp fed with probiotic supplemented feed for 21 days, in second phase challenged with White spot syndrome virus (WSSV) and their physiological responses were investigated for 2 days post-challenged. The probiotic bacteria Lactobacillus sps was added to the formulated basal diet at 5%, 10% and 15%concentration. Growth, immune response, antioxidant status of shrimp were evaluated. The results showed that dietary supplementation of probiotics in shrimp had significant (P<0.01) impact on growth. The treated shrimp groups showed significant increase in THC, percentage of phagocytosis and phenoloxidase enzyme activity. IgG, IgA, and IgM like substances in the haemolymph of treated shrimp were significantly increased when compared to control. Higher levels of these substances were observed in 10% treated shrimp than other two groups. The antioxidant enzymes like catalse and superoxide dismutase enzymes also significantly increased in probiotic treated shrimp when compared to control. In addition, dietary supplementation of all the three concentrations of Lactobacillus sps probiotic bacterium was effective in improving the resistance of shrimp against WSSV as they had higher THC, higher percentage of phagocytosis, phenoloxidase enzyme and immunoglobin like substances level.

Keywords: 
Antioxidant response, immune response, Probiotics, WSSV
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