Homology Modeling of Endoxylanase from Trichoderma pseudokoningii

Order of Publishing in Issue: 
Volume :10
Issue :3
July, 2016
Page No: 
Bandikari Ramesh, Seelam Naga Sivudu, Katike Umamahesh and Obulam Vijaya Sarathi Reddy*
Department of Biochemistry, Sri Venkateswara University, Tirupati - 517 502, India.

Endoxylanase is involved in the degradation of xylan to xylose. It majorly breaks down the hemicelluloses, which are the major components of the cell wall of the plants. In the present study the 3D modeling of endoxylanase from Trichoderma pseudokoningii (B0FXM0) was performed by comparing modeling approach using endo 1-4 β-xylanase from Trichoderma reesei (PDB ID: 1XYP) as template in modeler7V.7 program server. The best model was selected based on overall stereo-chemical quality (PROCHECK, proSA, Verified_3D), and also verified with Protein Structure Validation Suit (PSVS). The active sites of endoxylanase were identified in CastP server, which showed that the refined model has highly conserved residues.

Homology modeling, Modeller, Ramachandran plot, Xylanase
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