Growth inhibition and induction of apoptosis in estrogen receptor-positive and negative human breast carcinoma cells by Adenocalymma alliaceum flowers
AbstractAdenocalymma alliaceum (A. alliaceum)is well known for its traditional medicinal usesand as a substitute for garlic. The methanol extractfrom A. alliaceum flowers (AAF) wasinvestigated for its growth inhibitory activity onthe estrogen receptor positive MCF-7 andestrogen receptor negative MDA-MB231 breastcancer cells by MTT assay. Treatment of breastcancer cells with different concentrations of AAFresulted in dose dependent growth inhibition witha growth inhibitory concentration (GI50) of53.1±4.1 µg/mL in MCF-7 and 23.9±3.7 µg/mLin MDA-MB-231 cells. Treatment of breastcancer cells with AAF resulted in time-dependentsequence of events marked by apoptosis, asshown by translocation of phosphatidylserine andactivation of caspase-3. Analysis of data suggeststhat AAF exerts growth inhibition on both breastcancer cells through apoptosis induction, and thatit may contain potent anticancer secondarymetabolites valuable for application in drugproducts.
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