Glucose Regulated Protein 78 (GRP 78) as a Cytoprotection against Apoptosis in Small Cell Lung Carcinoma

Order of Publishing in Issue: 
9
Volume :8
Issue :2
April, 2014
Page No: 
185-191
Authors: 
R. Masalu[1]*, K.M.M. Hosea[1], M. Meyer[2] , S.L. Lyantagaye[1] and B. Ndimba[2]
Address: 
[1]Department of Mol Biology and Biotechnology, University of Dar es Salaam, P.O Box 35179, Dar es Salaam, Tanzania
Address: 
[2]Department of biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, Cape Town, South Africa
Email-ID: 
roseminner@yahoo.com

In a previous study, has been found that mushroom crude extract (Cantharellus sp) is able to induce apoptosis in human cervical cancer cell line (HeLa) and the resistance to Small Cell Lung Carcinoma (H157). To clarify the mechanism, we carried out comparative proteomics analysis between H157 and HeLa cell lines. Differentially expressed proteins were separated electrophoretically and identified by Matrix Assisted Laser Desorption Time of Flight Mass Spectrometry (MALDI-TOF-MS). Peptide tandem mass spectra were searched against a comprehensive data base containing known proteins derived from data bases. There were profound changes in 14 proteins related to mitochondrial function and oxidative stress. The results also showed up regulation of GRP78 in treated H157 cell line. Up regulation of GRP78 was further confirmed by western blotting analysis and it was suggested that GRP78 is responsible for apoptosis resistance of H157 cell line when treated with Cantharellus extract at low concentration.

Keywords: 
Glucose Regulated protein 78, Apoptosis, Cantharellus, Small Cell Lung Carcinoma cell line.
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