Gluco-oligosaccharides production from glucan of Leuconostoc mesenteroides NRRL B-742 by microwave assisted hydrolysis

Order of Publishing in Issue: 
8
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
405-411
Authors: 
Avishek Majumder[1], Anshuma Mangtani, Seema Patel, Rishikesh Shukla and Arun Goyal*
Address: 
Department of Biotechnology, Indian Institute of Technology Guwahati Guwahati 781 039, Assam, India
Address: 
[1] Present address: Department of System Biology, Enzyme and Protein Chemistry Group, Technical University of Denmark, Building 224, DK-2800 Kgs. Lyngby, Denmark

Abstract : The glucan composed of ? -(1?6) linkages with ? -(1?3) and ? -(1?4) branched linkages points was produced from Leuconostoc mesenteroides NRRL B-742. The glucan was hydrolysed by acid using microwave oven for production of gluco-oligosaccharides for time intervals of 2 min and 3 min and the optimum time period for oligosaccharide production was determined. The yield of oligosaccharides production from glucan was 2.5% for 2 min of hydrolysis time. The hydrolysed glucan was run on Sephadex G-10 column using FPLC and oligosaccharides produced were eluted and analyzed by MALDI Q-TOF mass spectrometer in MS/MS mode. The glucan sample hydrolysed by microwave for 2 min yielded the peak at ~527 m/z value showing the presence of 3 glucose units (m/z ~180) corresponding to the glucooligosaccharide with degree of polymerisation 3.

Keywords: 
Glucansucrase, Leuconostoc mesenteroides, Glucan, Gluco-oligosaccharide, MALDI-TOF.
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