Genotype and Allele Frequencies of DGAT 1 Gene in Indian Holstein Bulls

Order of Publishing in Issue: 
5
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
385-388
Authors: 
R. K. Patel, J. B. Chauhan, K. J. Soni, K. M. Singh
Address: 
R & D-Biotechnology, National Dairy Development Board, Anand- 388 001, India
Email-ID: 
rkpatel46@yahoo.com

Abstract : A Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the diacylglycerol oacyltransferase 1 gene (DGAT 1) in Indian Holstein Friesian (HF) bulls. Recent studies indicated that the DGAT 1 gene is responsible for fat production and found on centromeric region of bovine chromosome 14. Because of polymorphism, the DGAT 1 gene has two major genetic variants (K and A) which occurred due to an amino acid substitution in the candidate gene. The allele (K) encoding the lysine proved to be more efficient with regard to the milk fat synthesis is obviously useful markers for milk fat traits on which bull can be evaluated and selected for future breeding programmes. DNA samples were extracted from blood samples of 281 HF bulls stationed at various sperm stations across the country. A 411 bp fragment of DGAT 1 was amplified and digested with Cfr1 restriction enzymes. Two types of alleles K and A and three types of genotypes KK, AA and KA were observed in the study. The results indicated allelic frequency of K-allele (0.59) was higher compared to A-allele (0.41) for DGAT 1 in Indian HF population.

Keywords: 
DGAT1, HF, Milk fat, QTL, PCRRFLP, Genotype.
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