Expression of a bacterial chitinase (ChiB) gene enhances antifungal potential in transgenic Litchi chinensis Sonn. (cv. Bedana)

Order of Publishing in Issue: 
11
Volume :4
Issue :3
July, 2010
Page No: 
820-833
Authors: 
Das, D.K.* and Rahman, A.
Address: 
Post Graduate Department of Biotechnology, T.M.Bhagalpur, Bhagalpur-812007, India University,
Email-ID: 
dilipdas1@live.com

Abstract

Transgenic litchi harboring chitinase and gus genes were generated via Agrobacterium mediated transformation of zygotic embryos. Confirmation of the transgenic was done by PCR, RT-PCR, Southern blotting and by Western blot. Functional analysis of the transgenic was also attempted.

Keywords: 
Agrobacterium tumefaciens, Somatic embryogenesis, die back, leaf spots and blight, Transformation, Phomopsis sp.
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