Evaluation of curcumin as potential herbal agent for mitigation of nickel and chromium induced micronuclei in human blood cultures

Order of Publishing in Issue: 
Volume :2
Issue :4
October, 2008
Page No: 
567 -574
Rao M.V.,* Jhala D.D., Chettiar S. S. and Patel A.R.
Cytogenetic Division, Zoology Department, School of Sciences, Gujarat University, Ahmedabad - 380009

Chromium and nickel are widely used  ndustrial chemicals.Welders in India are inclinedto possible occupational chromium and nickel exposure. We have investigated the Genotoxicityexerted by nickel (Ni) in the form of nickel chloride (4.216 X 10-5M) and chromium (Cr) inthe form of potassium dichromate (1.36 X 10- 6M), alone and in combination and mitigatingeffect of curcumin (3.87 X 10-7M) was studied at two different exposure intervals (24 and 69 hours)in toxicant added human blood cultures. Evaluation of genotoxicity was done bymicronuclei (MN) assay. One thousand binucleate cells per group were analyzed.Statistical analysis was performed by one way ANOVA followed by Dunett’s test and percentageamelioration was calculated. Nickel and chromium proved to be more toxic in 69-hoursexposure than in 24 hours in induction of micronuclei. Curcumin supplementationexhibited significant (71%-92%) amelioration at both exposure intervals. Hence, curcumin has amitigating effect against micronuclei induced by metal salts due to its antioxidant and free radicalscavenging properties.

Nickel chloride, Potassium dichromate, Curcumin, Genotoxicity, Micronuclei Human blood cultures.
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