An Evaluation of Antioxidant Potential of Memecylon sisparense Gamble Leaf in Doxorubicin- Induced Cardiotoxicity in Mice

Order of Publishing in Issue: 
5
Volume :13
Issue :1
January, 2019 - March, 2019
Page No: 
41-49
Authors: 
Jaya Lakshmi Uppu[*#] Veerabhadra Swamy Challa[*], Dr.Ganga Modi Naidu Vegi[*] Dr.Asha S[#]
Address: 
[*]Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, (NIPER) Hyderabad, INDIA
Address: 
[#]Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research University, Vadlamudi, Guntur, INDIA
Email-ID: 
sai848@gmail.com

Memecylon species has been reported for anti-cancer, anti-inflammatory, anti-hypertensive activities but Memecylon sisparense Gamble (MSG) activity is not yet reported. The present study is aimed for the first time to investigate on the antioxidant potential of MSG leaf ethyl acetate extract (EAE) on Doxorubicin (DOX) induced cardiotoxicity in mice model. The antioxidant activity was studied by total phenolic, flavonoid content, DPPH assay. Swiss albino male mice were treated with MSG leaf EAE for 9 consecutive days with DOX 15mg/kg, i.p on day 7 to induce cardiotoxicity. ECG was recorded after 48h DOX administration, collected blood sample for CK-MB, LDH, AST, ALT and heart tissues for studying antioxidant parameters SOD, CAT, GSH, NO, MDA, histopathology. MSG leaf EAE showed good antioxidant activity with 93.67% DPPH radical inhibition at 250 μg/mL. DOX significantly increased heart/bodyweight ratio along with CK-MB, LDH, AST, ALT levels in plasma, whereas treatment group significantly decreased the levels. In ECG recording, MSG leaf EAE decreased the ST segment which was elevated in DOX treated animals. In cardiac tissue, our extract decreased MDA, NO as DOX induced antioxidant markers with an increase in SOD, CAT and GSH levels thereby showing protective mechanism through inhibition of oxidative stress. This is the first report that elucidated the antioxidant potential of MSG leaf EAE. Our results suggested that MSG leaf pre-treatment has an important therapeutic benefit during DOX therapy by inhibiting oxidative stress there by inhibiting lipid peroxidation, enhancing the cardio protective activity.

Keywords: 
Antioxidant, Cardiotoxicity, Doxorubicin, Electrocardiogram, Histopathology.
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