Evaluation of Antimicrobial Activity of Emblica officinalis against Skin Associated Microbial Strains

Order of Publishing in Issue: 
6
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
355-366
Authors: 
Lovey Sharma[1]* and Ram Kumar Pundir[1]
Address: 
[1]Department of Biotechnology, Ambala College of Engineering and Applied Research, Devsthali (near Mithapur), P.O. Sambhalkha-133101, Ambala (Haryana)-India

The present study was undertaken to access antimicrobial activity of fruit extracts of Amla (Emblica officinalis) against skin associated microorganisms. The antimicrobial activity of five different solvents viz. methanol, ethanol, distilled water, chloroform and petroleum ether against Gram positive, Gram negative bacteria and yeast namely Propionibacterium acne, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans was assessed by using agar well diffusion method. The methanolic extract of Emblica officinalis showed maximum zone of inhibition, aqueous extract was most effective against S. aureus and ethanolic extract was most effective against C. albicans. Comparison of antimicrobial activity of Emblica officinalis extracts with antibiotics revealed that Emblica officinalis methanolic extract had maximum effective antimicrobial activity against all tested microorganisms. MIC and MBC of methanolic extract of Emblica officinalis against the microbial strains was ranged between 0.50 to 0.03125mg/ ml. The synergistic interaction of Emblica officinalis with antibiotics (Gentamicin, Amikacin and Clotrimazole) indicated much better results as compared to antibiotics susceptibility pattern alone. Phytochemicals analyses showed the presence of Alkaliods, Saponins, Glycosides, Proteins, Phenols and Phytosterols. The compounds identified by GC-MS analysis had been useful as skin conditioning agent. The present study reflects a hope for the development of novel agents of biomedical importance.

Keywords: 
Antimicrobial activity, Emblica officinalis fruit, GC-MS analysis, Phytochemicals, Skin disease.
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