Estimation of Biochemical Activities of Microbial Load Isolated from the Frozen Semen of HF and HF Crossbred Cattle Bulls

Order of Publishing in Issue: 
Volume :6
Issue :3
July, 2012
Page No: 
Dhruti Y. Patel and Rajesh K. Patel
Ashok and Rita Patel Institute of Integrated study and Research in Biotechnology and Allied Sciences (ARIBAS), New Vallabh Vidyanagar- 388 121 (Gujarat) INDIA


Total 30 French mini straws (0.25ml) of frozen semen of HF and HF crossbred cattle bulls were randomly collected from one of the frozen semen banks for evaluation of microbial load using the standard plate count (SPC) method using nutrient agar plate. These plates were incubated at 37oC for 72 hrs and examined for growth. The average colony count was calculated and bacteria were also identified as Gram positive and Gram negative. A total of 10 biochemical tests were performed to characterize the isolates. Antibiotic sensitivity test was also performed to test the sensitivity against Ampicillin, Erythromycin, Gentamycin and Spectinomycin. The results indicate that eleven samples out of 30 (36.6%) were found positive for various bacterial isolates. In most samples the microbial load was found below the Bureau of Indian Standard (BIS). However one sample (3.33%) was found with fungal infection. Both the Gram positive and Gram negative bacteria were found in these samples. Results of biochemical properties of bacterial isolates are summarized. Each isolates are varying in their biochemical profile. The results of antibiotic sensitivity pattern in bacterial isolates were also summarized. All bacterial isolates exhibited variable pattern against Ampicillin, Erythromycin, Gentamycin and Spectinomycin. The concentrations of antibiotics were 10μg, 10μg, 10μg and 100 μg respectively. Among all these antibiotics all bacterial isolates are resistance against Ampicillin. The article describes detailed investigation of microbial load in frozen semen of HF and HF crossbred cattle bulls

Holstein Cattle bull, frozen semen, microbial load, Biochemical tests, microorganism,
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