Enhanced Production of Glutathione from Saccharomyces Cerevisiae using Metabolic Precursor and Purification with New Approach

Order of Publishing in Issue: 
12
Volume :6
Issue :2
April, 2012
Page No: 
241-254
Authors: 
Parbatsingh Rajpurohit, Ashwini Tilay, Shrikant Survase and Uday Annapure*
Address: 
Food Engineering and Technology Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India

Abstract
This paper reports on the enhanced fermentative production of glutathione by Saccharomyces cerevisiae NCIM 3454 using a statistical approach and its successive purification by alternative technique. In the first step, one factor at-a-time method was used to examine the effect of carbon sources, nitrogen sources and pH on glutathione (GSH) production. Subsequently, statistical mathematical model was used to identify the optimum concentrations of the key nutrients for higher GSH production. Glutathione production increased significantly from 55.28 to 148.45 mg/L when Saccharomyces cerevisiae NCIM 3454 was cultivated using optimized medium, as compared to basal medium. Further, glutathione production was considerably increased to 163.12 mg/L by using cysteine amino acid as one of the metabolic precursor. In this study aqueous two phase system (ATPS) was found to be most useful technique to abolish contaminating proteins in glutathione purification. Further enhanced purification was carried out by adsorption chromatography (ion exchange) using a variety of Amberlite resins.

Keywords: 
Glutathione, Saccharomyces cerevisiae, fermentation, precursor, chromatography.
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