Enhanced L-lysine production through chemical mutagenesis in Corynebacterium glutamicum MTCC 25069

Order of Publishing in Issue: 
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
Ramesh Malothu
School of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kakinada - 533003, A.P, India

Lysine is one of the most commercially available amino acid, which is chiefly used as a feed additive, human medicine and as a dietary supplement. In the present day, Corynebacterium glutamicum is the most popularly known microorganism for the industrial biosynthesis of lysine. The purpose of this study was to improve the yield of lysine production by chemical mutagenesis. In this study the culture conditions were optimized and the maximum growth was observed at 37p C, pH-7.4 for 72 hours. The chemical mutagen, N Ethyl N Nitroso Urea (ENU) was used to develop the auxotrophic mutants by treating the wild type strain with different concentrations of ENU (i.e.,35mM, 50mM, 70mM, and 100mM) at different time periods (i.e.,0, 5, 10, 15, 20, and 25 mins). The obtained mutants were then inoculated in seed culture medium enriched with methionine and threonine and all the mutants obtained were screened for lysine concentrations. The most potent auxotrophic mutant developed in this study produced 30.2 g/l lysine when the culture was exposed to ENU of 70mM concentration for 10 minutes.

L-lysine, Corynebacterium glutamicum, N Ethyl N NitrosoUrea (ENU), mutagenesis, auxotrophs, seed culture medium.
PDF296.19 KB

To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.



Upcoming 0 events

  • No upcoming events available

User login