Enhanced L-lysine production through chemical mutagenesis in Corynebacterium glutamicum MTCC 25069

Order of Publishing in Issue: 
5
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
279-288
Authors: 
Ramesh Malothu
Address: 
School of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kakinada - 533003, A.P, India

Lysine is one of the most commercially available amino acid, which is chiefly used as a feed additive, human medicine and as a dietary supplement. In the present day, Corynebacterium glutamicum is the most popularly known microorganism for the industrial biosynthesis of lysine. The purpose of this study was to improve the yield of lysine production by chemical mutagenesis. In this study the culture conditions were optimized and the maximum growth was observed at 37p C, pH-7.4 for 72 hours. The chemical mutagen, N Ethyl N Nitroso Urea (ENU) was used to develop the auxotrophic mutants by treating the wild type strain with different concentrations of ENU (i.e.,35mM, 50mM, 70mM, and 100mM) at different time periods (i.e.,0, 5, 10, 15, 20, and 25 mins). The obtained mutants were then inoculated in seed culture medium enriched with methionine and threonine and all the mutants obtained were screened for lysine concentrations. The most potent auxotrophic mutant developed in this study produced 30.2 g/l lysine when the culture was exposed to ENU of 70mM concentration for 10 minutes.

Keywords: 
L-lysine, Corynebacterium glutamicum, N Ethyl N NitrosoUrea (ENU), mutagenesis, auxotrophs, seed culture medium.
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