Enhanced Hyaluronic Acid Production by a Mutant Strain, 3523-7 of Streptococcus zooepidemicus

Order of Publishing in Issue: 
12
Volume :5
Issue :4
October, 2011
Page No: 
1473-1479
Authors: 
K. Jagadeeswara Reddy[1.2], K.T. Karunakaran [2]* and K.R.S. Sambasiva Rao [1]
Address: 
[1] Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
Address: 
[2] MM Biolabs Private Limited, 274 Fortuna Glade B3/A, VB Layout, Bangalore, India

Abstract
Hyaluronic acid (HA) is a hydrated gel and comprises repeating units of glucuronic acid and N-acetylglucosamine. HA has been of considerable interest due to its clinical application. This study reports the optimization of fermentation conditions for HA production by Streptococcus zooepidemicus 3523-7, a mutant derived from MTCC 3523. In shake flask fermentation, CDM containing 2% glucose and 1.5% yeast extract at pH 7.2 and temperature 36oC with 1% inoculums favored maximum HA production by the strain 3523-7. Enhanced HA production (1.89 g/l) by 3534-7 was seen when the fermentation was carried out in a 10 L bioreactor under optimal conditions such as 400 rpm and aeration 0.6 vvm.

Keywords: 
Hyaluronic acid production, fedbatch fermentation, Streptococcus ooepidemicus mutant strain 3523-7.
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