Enhanced artemisinin production by cell cultures of Artemisia annua

Order of Publishing in Issue: 
12
Volume :2
Issue :2
March, 2008
Page No: 
12-128
Authors: 
Ashish Baldi* 1 and V.K. Dixit 2
Address: 
1 Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Address: 
2 Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar (M.P.) 470003, India
Email-ID: 
baldiashish@gmail.com

Artemisinin, an endoperoxide containing sesquiterpene lactone from Artemisia annua, hasproven very effective in treating drug resistant cases of malaria and cancer. To counter thepresent low content in leaves and uneconomical chemical synthesis, alternate ways to produceartemisinin have been sought. Inspite of extensive work in this area, artemisinin remainselusive in dedifferentiated and differentiated cultures of A. annua. This work reports the firstsuccessful approach for production of artemisinin by cell cultures of A. annua cultures of Indianvariety of A. annua. Various precursors of terpenoid biosynthesis by isoprenoid pathwaywere incorporated to study their influence on artemisinin biosynthesis. Artemisinin contentwas maximally increased by 2.0 times, in comparison to control, when mevalonic acid (50mg/L) was added as precursor. Various biotic and abiotic elicitors were also tested at differentconcentration. A maximum increase of 3.47 times in artemisinin accumulation was attained whenmethyl jasmonate (5 mg/L) was added. Based on these results, an integrated bioprocess forproductivity enhancement of artemisinin was developed. A maximum artemisininaccumulation of 96.8 mg/L artemisinin was produced on supplementation of mevalonic acidand methyl jasmonate as selected precursor and elicitor respectively, which was 4.79 times higherin productivity than control callus cultures (20.2 mg/L).

Keywords: 
Artemisinin, Artemisia annua, Hairy roots, Agrobacterium rhizogenes
AttachmentSize
121-128.pdf433.99 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login