AbstractThe Present study reveals the effect ofLactobacillus delbrucekii cells immobilized invarious matrices for the production of opticallypure L(+) lactic acid. Functionalized alginatematrices were effective and suitable for higher L(+) lactic acid yields compared to other matrices.Repeated batch fermentation showedproductivity of 1.74, 1.44, 1.48 and 1.52 Y p/swith functionalized alginate, Ca-alginate, KCarrageenan,and glass beads, respectively. L.delbrucekii cells were immobilized in naturaland functionalized alginate beads. The scanningelectron microscopic studies showed increase inentrapped microbial cell biomass in modifiedimmobilized beads compared to other matrices.These modified alginate beads showed enhancedstability and selectivity towards L (+) lactic acidproduction in higher yields with an enantiomericselectivity of 99% and low by-productproduction.