Effect of Physical Pre-treatment on Cellulases Production by Cladosporium sp. NCIM 901 in Submerged Fermentation using Bagasse as a Substrate

Order of Publishing in Issue: 
3
Volume :7
Issue :2
April, 2013
Page No: 
603-614
Authors: 
Poludasu Rama Mohan, Bathal Vijaya Kumar and Obulam Vijaya Sarathi Reddy*
Address: 
Department of Biochemistry, Sri Venkateswara University, Tirupati-517 502, India
Email-ID: 
ovsreddy@yahoo.com

The production of cellulases (filter paper activity, endoglucanase and β-glucosidase) by Cladosporium sp. NCIM 901 on different media in shake flask culture was compared. The culture filtrate of the organism exhibited highest total cellulolytic activity and extracellular protein content on 7 days interval during the course of its growth on Mary Mandel’s mineral medium supplemented with 1% cellulose compared to other media. Various soluble carbon sources were tested in this study, cellulose supported the maximum production of cellulolytic enzymes (1.64, 0.75 and 1.1 U/ml of FPase, CMCase and β-glucosidase). Enhanced production of cellulolytic enzymes was observed when KNO3 was used as nitrogen source (1.71, 0.93 and 1.23 U/ml of FPase, CMCase and β-glucosidase). Among the lignocellulosic materials, sugarcane bagasse increased cellulolytic enzymes production (1.94, 0.95 and 0.98 U/ml of FPase, CMCase and β-glucosidase) and the next best was saw dust. Maximum yield of cellulolytic enzymes were observed when bagasse was physically pretreated (1.972, 1.103 and 1.112 U/ ml of FPase, CMCase and β-glucosidase) and the reducing sugar concentration of 0.52 mg/ml was obtained.

Keywords: 
Cladosporium sp. NCIM 901, Celllulolytic enzymes, FPase, CMCase, β- glucosidase, Pretreatment.
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