Effect of Growth regulators, Sucrose and Glutamine on Shoot Regeneration from Node Derived Callus Cultures of Rubber yielding species - Parthenium argentatum

Order of Publishing in Issue: 
5
Volume :5
Issue :1
January, 2011
Page No: 
1004-1010
Authors: 
A. Maruthi Rao[1], I. Sampath Kumar[1], P. Sri Laxmi [2], P. Maheshwari Rao [2], P. Hima Kumari [2], S. Anil Kumar [2] D. Madhavi [2], E. Venu Madhav [3] and P. B. Kavi Kishor [2]*
Address: 
[1]Plant Biotechnology Research Lab, Department of Botany, Telangana University, Nizamabad 503 322, India
Address: 
[2] Department of Genetics, Osmania University, Hyderabad 500 007, India
Address: 
[3] Department of Biotechnology, Achayra Nagarjuna University, Nagarjuna Nagar, - 522 510, India
Email-ID: 
pbkavi@yahoo.com

The nodal explants of guayule (Parthenium argentatum Gray) when cultured on Murashige and Skoog’s  (MS) medium fortified with different concentrations of kinetin (KN), 6- benzylaminopurine (BAP), 2,4- dichlorophenoxyacetic acid (2,4-D), 2,4-D + BAP, napthaleneacetic acid (NAA) and NAA + BAP produced callus tissues and few shoots. The percent frequency of callus was around 90% with 0.5 mg/l BAP and NAA. The high frequency shoot regeneration from the node derived callus of guayule was observed when MS medium was supplemented with BAP, NAA + BAP with varying concentrations of sucrose. Sucrose at 3- 4% levels increased the number of shoots formed (12-15) per 250 mg of callus. Glutamine at 200 mg/l level further enhanced the number of shoots formed (22-25) per callus mass. The percent frequency of shoot regeneration from callus tissues was high (88%) when the medium was fortified with 0.1 mg/l NAA + 2.5 mg/l BAP, 200 mg/l glutamine and 2% sucrose. The ability of the callus tissues of guayule remained more or less same for shoot regeneration over a period of 200 days; but decreased thereafter. Shoots rooted with 15 to 75% frequency and the whole plants when transferred to pots survived with 60% frequency.

Keywords: 
Callus cultures, plant regeneration, guayule (Parthenium argentatum Gray).
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