Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers
Berberine, an isoquinolene alkaloid, together with its related analogs protoberberineand palmatine were detected in cell suspension cultures derived from leaf explants of Tinosporacordifolia. Maximum production of biomass (9.9 g dry cell wt/l) was noticed on day 36 whileberberine was highest (5.5 mg/g dry weight) at day 24. Media pH, plant growth regulators andcarbon sources were optimized for biomass and accumulation of berberine in Linsmaier andSkoog’s medium. Out of 8 cell lines selected, only one line accumulated high berberine (13.9mg/g dry wt) in 4-weeks. While cell aggregates of 500 ?m in diameter promoted production ofbiomass (9.6 g dry cell wt/l), larger cell aggregates (above 500 ?m) favoured berberineaccumulation with 3.8 mg/g dry weight of tissue. The yield of berberine in cell suspensions ofTinospora was 5-14-folds higher than that of intact plants.
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