Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers

Order of Publishing in Issue: 
Volume :2
Issue :2
March, 2008
Page No: 
B. Rama Rao1, D. Vijay Kumar1, R. Naga Amrutha2, N. Jalaja2, K. Vaidyanath2, A. Maruthi Rao3, Srinath Rao4 Rathnagiri Polavarapu5 and P. B. Kavi Kishor2
1Natural Products Laboratory, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 007, India
2Department of Genetics, Osmania University, Hyderabad 500 007, India
3Department of Botany, Telangana University, Nizamabad 503 002, India
4Department of Botany, Gulbarga University, Gulbarga 585 106, India
5Genomix Molecular Diagnostics Pvt. Ltd., Balanagar, Hyderabad 500 072, India

Berberine, an isoquinolene alkaloid, together with its related analogs protoberberineand palmatine were detected in cell suspension cultures derived from leaf explants of Tinosporacordifolia. Maximum production of biomass (9.9 g dry cell wt/l) was noticed on day 36 whileberberine was highest (5.5 mg/g dry weight) at day 24. Media pH, plant growth regulators andcarbon sources were optimized for biomass and accumulation of berberine in Linsmaier andSkoog’s medium. Out of 8 cell lines selected, only one line accumulated high berberine (13.9mg/g dry wt) in 4-weeks. While cell aggregates of 500 ?m in diameter promoted production ofbiomass (9.6 g dry cell wt/l), larger cell aggregates (above 500 ?m) favoured berberineaccumulation with 3.8 mg/g dry weight of tissue. The yield of berberine in cell suspensions ofTinospora was 5-14-folds higher than that of intact plants.

Berberine, suspension cultures, Tinospora cordifolia.
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