Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers

Order of Publishing in Issue: 
5
Volume :2
Issue :2
March, 2008
Page No: 
49-56
Authors: 
B. Rama Rao1, D. Vijay Kumar1, R. Naga Amrutha2, N. Jalaja2, K. Vaidyanath2, A. Maruthi Rao3, Srinath Rao4 Rathnagiri Polavarapu5 and P. B. Kavi Kishor2
Address: 
1Natural Products Laboratory, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 007, India
Address: 
2Department of Genetics, Osmania University, Hyderabad 500 007, India
Address: 
3Department of Botany, Telangana University, Nizamabad 503 002, India
Address: 
4Department of Botany, Gulbarga University, Gulbarga 585 106, India
Address: 
5Genomix Molecular Diagnostics Pvt. Ltd., Balanagar, Hyderabad 500 072, India
Email-ID: 
pbkavi@yahoo.com

Berberine, an isoquinolene alkaloid, together with its related analogs protoberberineand palmatine were detected in cell suspension cultures derived from leaf explants of Tinosporacordifolia. Maximum production of biomass (9.9 g dry cell wt/l) was noticed on day 36 whileberberine was highest (5.5 mg/g dry weight) at day 24. Media pH, plant growth regulators andcarbon sources were optimized for biomass and accumulation of berberine in Linsmaier andSkoog’s medium. Out of 8 cell lines selected, only one line accumulated high berberine (13.9mg/g dry wt) in 4-weeks. While cell aggregates of 500 ?m in diameter promoted production ofbiomass (9.6 g dry cell wt/l), larger cell aggregates (above 500 ?m) favoured berberineaccumulation with 3.8 mg/g dry weight of tissue. The yield of berberine in cell suspensions ofTinospora was 5-14-folds higher than that of intact plants.

Keywords: 
Berberine, suspension cultures, Tinospora cordifolia.
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