Effect of different growth regulators on in vitro multiplication of strawberry (Fragaria × ananassa Duch.) cv. Camarosa through runner tip was studied with various combinations and concentrations IAA, BAP & Kinetin and IBA & NAA hormones using Murashige and Skoog media (MS). MS media with 0.5 μM IAA + 2.5 μM kinetin took minimum days (11.67) for shoot proliferation and produced maximum number of shoots/ culture (12.67) followed by 0.1 μM IAA + 2.0 μM kinetin. Application of IBA (2 mg/l) in MS media initiated maximum roots in minimal time (7.33). Maximum mean number of roots/ explant (9.33) and length of roots (6.17 cm) was also recorded in MS + IBA (2 mg/ l) followed by MS + IBA (1 mg/ l) media. The rooted plants transferred in cocopeat + perlite + vermicompost (3:1:1) showed maximum transplanting success (94.33%) while minimum from S6 (63.00%). The outcome of the study is useful for in vitro multiplication and genetic engineering work in strawberry in future.