Direct Organogenesis and Plant Regeneration from Cotyledons of a Multipurpose Tree, Acacia mangium Willd.
Abstract : Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with IBA (8.0 μM) was found best for rooting of in vitro shoots. The rooted plantlets were then transferred to small pots containing a sterilized mixture of sand, garden soil and compost (1:1:1) for acclimatization where plantlets grew well with 65% survival rate.