Determination of tramadol in capsules by high performance thin layer chromatography - densitometry

Order of Publishing in Issue: 
Volume :2
Issue :2
July, 2008
Page No: 
421 -425
Venkateshwarlu K1,3, Reddy YN3*, Srisailam K2, Rajkumar V2 and Pai MG1
1Department of Pharmaceutical Analysis, Goa College of Pharmacy, Panaji, Goa, India
2Vaagdevi College of Pharmacy, Ramnagar, Hanamkonda, Warangal, AP, India, 3University College of Pharmaceutical Sciences, Kakatiya University, Warangal, AP, India

A simple, sensitive and reproducible high performance thin layer chromatographic methodfor the determination of tramadol in capsules is developed using silica gel plates with fluorescentindicators. The system is equipped with an automated sample applicator and the detectionwas performed at 254 nm by using HPTLC. The mobile phase consists of methanol and ammoniain the ratio of 19:1. The retention factor for tramadol is 0.67. The limit of detection oftramadol was found to be 13 ?g/ml and the limit of quantitation is 15 ?g/ml. The method showsgood linearity in the range of 18 to 100 ?g/ml (R2 = 0.993). The intra and inter day assay(n=5)variations in the linear range are less than 2%. A pharmaceutical capsule productcontaining tramadol was analyzed to test the applicability of new method. The percentage oftramadol in the capsules was found to be from 93.5 to105

Tramadol, HPTLC of Tramadol, Tramadol capsules, Densitometry
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