Correct identification of wood-inhabiting fungi by ITS analysis

Order of Publishing in Issue: 
4
Volume :1
Issue :1
March, 2007
Page No: 
41-61
Authors: 
Annette Naumann #, Mónica Navarro-González #, Olivia Sánchez-Hernández #, Patrik J. Hoegger # and Ursula Kües #*
Address: 
Georg-August-University Göttingen Büsgen-Institute Section Molecular Wood Biotechnology and Technical Mycology Büsgenweg 2, D-37077 Göttingen
Email-ID: 
ukuees@gwdg.de

In this study, we established ITS sequences from 20 strong wood degraders from different fungalgenera and from 15 strains of the coprini which are occasionally found on wood. These sequenceswere used in blast searches to evaluate their species identity. ITS sequences confirmed for 25 strains previous morphological species determination.ITS sequences from Coprinopsis scobicola were determined for the first time from DNA of two different strains. Finally, eightstrains were found assigned to a wrong species or assignments were uncertain. We present problemsencountered in fungal identification by molecular data, such as difficulties in previous morphologicalidentification, occurrence of contaminations or mixing-up of strains in fungal culture collections, and wrong entries in the NCBIGenBank sequence dataset. A long-term target of ITS sequencing of wood-inhabiting fungi is to establish a specific database that can be eitherused for blast searches or in ITS barcoding for species identification. We present first studies in ITS barcoding of coprini.

Keywords: 
Wood degraders, Coprinoid mushrooms, misidentifications, phylogenetic analysis, barcoding
AttachmentSize
41-61.pdf1.3 MB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login