Comparative Efficacy of Selected Disinfectants against Pathogenic Bacteria Isolated from Hospital Fomites and Disposal Wastes

Order of Publishing in Issue: 
3
Volume :13
Issue :1
January, 2019 - March, 2019
Page No: 
26-35
Authors: 
Deepti Gulati[1]* and Devirupa Chakraborty[2]
Address: 
[1]Department of Biotechnology, Dolphin (PG) Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, India
Address: 
[2]Department of Biotechnology, Dolphin (PG) Institute of Biomedical & Natural Sciences, Dehradun, Uttarakhand, India

Prevention of health care associated infection has become an alarming concern and challenge for clinicians. This can be achieved by proper infection control practices, appropriate use of sterilization and disinfection procedures. In the current study eight bacteria were isolated from hospital wastes, collected from Government Hospital, Prem Nagar, Dehra Dun, by standard isolation methods. All the bacterial isolates were characterized by staining and biochemical tests up to the genus level. Among them two were grampositive while six were gram-negative bacteria. The isolates were identified to be Pseudomonas sp., Klebsiella sp., Micrococcus sp., Enterococcus sp., Alcaligenes sp., Serratia sp., Proteus sp. and Escherichia sp. These isolates were subjected to bactericidal activity testing against three commercially available disinfectants namely Formalin (Formaldehyde), Pursue (Dimethyl ammonium chloride) and Phenyle (Phenol). Efficacies of these disinfectants were evaluated by Minimum Inhibitory Concentration using agar diffusion method. Among the three disinfectants, Formalin and Pursue showed better activity for both Gram-negative (Zone of Inhibition: 43 mm and 46 mm respectively) and Gram–positive bacteria (Zone of Inhibition: 38 mm and 32 mm respectively). Phenyle, mostly used in our hospitals, was found to be less effective for all the isolates (Zone of Inhibition: 25 mm for Gramnegative and 17 mm for Gram-positive bacteria). Minimum Inhibitory Concentration of each disinfectant was also determined and was found to be best in Pursue (at dilution 1:512) followed by Formalin (1:64) and Phenyle (1:4).

Keywords: 
Disinfection, Formalin, Health-care, Phenyle, Pursue.
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