Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats

Order of Publishing in Issue: 
10
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
327-339
Authors: 
Veerappan Ramanathan[1], GV Swarnalatha[2], B. Manimegalai[1], A. Dominic Amalraj[1], Senthilkumar Rajagopal[2,*]
Address: 
[1]Department of Biochemistry, Enathi Rajappa Arts and Science College, Pattukkottai, TN, India
Address: 
[2]Department of Biochemistry, Rayalaseema University, Kurnool, AP, India
Email-ID: 
senthilanal@yahoo.com

Hypertension is one among the important factors that causes cardiovascular disorders. Nùnitro- L-arginine methyl ester (L-NAME) induces hypertension by blocking nitric oxide (NO) synthesis. Aim of present study was to investigate the effects of chrysin is one of major flavnoids, on L-NAME-induced hypertensive rats. Induces hypertension in adult male wistar rats weighing 180-220 g by oral treated of L-NAME (40 mg/kg/ day) dissolved in drinking water daily for 8 weeks. Experimental rats were oral treated with chrysin (25 mg/kg b.w). Both the systolic and diastolic blood pressure of control and experimental rats were measured by tail cuff plethysmography system. In our studies results showed an increase in the levels of systolic and diastolic blood pressure, heart, liver, kidney, body weight, plasma, and aortic Angiotension converting enzymes (ACE), Sodium (Na+), Chloride (Cl-) levels in LNAME treated rats. At the same time in L-NAME treated rats, there was a decrease in the levels of potassium (K+), Plasma and heart- aortic nitrite/ nitrate level, mitochondrial enzymes in liver such as Isocitrate dehydrogenase (ICDH), áketoglutarate dehydrogenase (á-KGDH), Succinate dehydrogenase (SDH) and Malate dehydrogenase (MDH). Chrysin treatment prevented the increase in systolic and diastolic blood pressure in the L-NAME-treated rats. Blood pressure (BP) reduction was interrelated with a reduction in Na+, Cl-, ACE activity and increased K+, plasma and heart, aortic nitrite/nitrate levels. In contrast, L-NAME had opposite effects on mitochondrial liver enzymes, electrolytes, ACE and NO by treatment of chrysin. Hence, the present findings might suggest that chrysin improve the balance between circulating nitric oxide and rennin-angiotensin system and beneficial effects on cardiovascular tissue through its ACE inhibitor activity.

Keywords: 
Angiotensin II, Chrysin, hydroxyproline, β - ketoglutarate dehydrogenase, nitric oxide, renin-angiotensin system.
AttachmentSize
PDF1.88 MB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

Upcoming 0 events

  • No upcoming events available

User login