Characterization of Myostatin Gene and Identification of SNPs for Diversity Analysis

Order of Publishing in Issue: 
6
Volume :1
Issue :1
March, 2007
Page No: 
70-78
Authors: 
S. T. Bharani Kumar[1], [2], Neeraj Dilbaghi[2], S P S Ahlawat[3], Bina Mishra[1], M. S. Tantia[1]* and R. K. Vijh[2]
Address: 
[1]National Bureau of Animal Genetic Resources, Karnal 132001, India.
Address: 
[2]Guru Jambheshwar University of Science and Technology, Hisar
Address: 
[3]Indian Veterinary Research Institute, Izatnagar.
Email-ID: 
mstantia@yahoo.com

In this present study we have sequenced the complete Myostatin gene with 3 exons, two introns, promoter region, 5’ and 3’ UTR region.We sequenced the entire gene by designing a set of 20 primers. Ten individuals from 5 breeds ofIndigenous chicken were sequenced and the sequences obtained were aligned to develop aconsensus sequence. We compared the sequences obtained among themselves, with Red JungleFowl and Chinese fowl. We estimated three genetic distances and constructed the phylogenetic tree using NJ method. Theindigenous chicken joined together first followed by Red Jungle Fowl while the Chinese fowl wasdistinct. The indigenous gene sequence was similar among the various chicken breeds and might have been evolved from RJF following aseparate course than the evolution of Chinese chicken.

Keywords: 
Characterization of Myostatin Gene and Identification of SNPs for Diversity Analysis
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