Characterization and Evaluation of Antibacterial, Antioxidant and Cytotoxicity of synthesised silver nanoparticles (AgNps) using chloroform crude callus extracts of Wrightia tinctoria (Roxb.)

Order of Publishing in Issue: 
7
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
275-285
Authors: 
Keerthi Priya Yadala and Asha S *
Address: 
Department of Biotechnology, VFSTRU, Vadlamudi, Guntur Dist., A.P
Email-ID: 
sai848@gmail.com

The present work established that the chloroform callus extract taken from the leaf midrib of in vitro grown seedling of Wrightia tinctoria (Roxb.) is very efficient for the synthesis of AgNPs. The produced silver nanoparticles were preliminarily conformed by UV Visible spectroscopy, SEM, TEM, FTIR spectroscopy and Energy Dispersion Spectrum. UV-Vis spectroscopy analysis revealed the peak at 425nm which corresponds to the surface plasmon resonance of AgNPs. The uniform spherical shape and size was detected by SEM and TEM. The size and stability of AgNps were detected using Dynamic light scattering (DLS), Zeta potential. The synthesised silver nano particles were evaluated for its antibacterial by agar well diffusion, antioxidant activities by DPPH assay and cytotoxicity on MCF-7 and HEK 29 were tested by MTT assay. The result indicated decrease in cell viability and cell growth inhibition. The present study highlighted the possibility of utilizing nanoparticles synthesised from in vitro derived callus of WTR for animal or human applications as it enhanced antibacterial, antioxidant activity and Cytotoxicity.

Keywords: 
WTR leaf midrib callus, Silver nanoparticles, Bio-reductant, DPPH inhibition, Antibacterial activity, Antioxidant and Cytotoxicity.
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