Synthesis of Silver Nanoparticles from Different Plant Leaf Extracts and Its Analysis using UV-Spectroscopy

Order of Publishing in Issue: 
9
Volume :10
Issue :3
July, 2016
Page No: 
286-289
Authors: 
Lavanya Kakarla[1] and Chakravarthy Rama[2] *
Address: 
[1],[2]Department of Biotechnology, K L University, Green Fields, Vaddeswaram – 522502, Guntur, Andhra Pradesh, India

The synthesis of silver nanoparticles has attracted much attention because their unique shape-dependent optical, electrical, and chemical properties have potential applications in nanotechnology. Silver nanoparticles are used in photographic reactions, catalysis, and chemical analysis. An attempt has been made to study the formation of the metal nanoparticles by reduction of the aqueous metal ions during exposure to the broth of boiled sunflower leaves may be easily followed by UV–vis spectroscopy.

Keywords: 
UV visible spectroscopy, Helianthus annuvus, Coriander sativum, Nanoparticles, Nanotechnology

Effect of Growth Regulators on Regeneration Through Leaf and Stem Derived Callus in Physalis minima Linn.

Order of Publishing in Issue: 
8
Volume :10
Issue :3
July, 2016
Page No: 
280-285
Authors: 
Sandhya H. and Srinath Rao*
Address: 
Plant Tissue Culture and Genetic Engineering Laboratory Department of Post Graduate Studies and Research in Botany, Gulbarga University Kalaburagi- 585106 Karnataka, India.

Physalis minima L. is a medicinal herb which belongs to the family Solanaceae.

Keywords: 
Physalis minima, leaf, stem, callus, organogenesis

Evaluation of Incubation Parameters in a TUNEL assay for Detecting Apoptotic cells in Cumulus Oocyte Complexes and in vitro produced early Embryos of Buffalo

Order of Publishing in Issue: 
7
Volume :10
Issue :3
July, 2016
Page No: 
274-279
Authors: 
Asit Jain[1], Tripti Jain[2], G K Sachdeva, Sachinandan De and Tirtha Kumar Datta*
Address: 
Animal Genomics Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal-132001, Haryana
Address: 
[1]Currently at Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, CGKV, Anjora, Durg, Chhattisgarh, India
Address: 
[2]Currently at Animal Biotechnology Centre, CGKV, Durg, Chhattisgarh, India
Email-ID: 
tirthadatta@gmail.com

Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL) is used widely for detecting the apoptotic status in biological cells of diverse origin. Reproducing the TUNEL procedure with specialized cells like cumulus oocyte complexes (COCs) poses technical challenge in view of mammalian oocytes which undergo very dynamic transitions in the course of oocyte maturation within a short time.

Keywords: 
Apoptosis, Cumulus Cells, Buffalo, TUNEL, Fixative

Isolation and Characterization of Extracellular Lipase Producing Bacterial Isolates From Effluent Waste of Paint Industry

Order of Publishing in Issue: 
6
Volume :10
Issue :3
July, 2016
Page No: 
261-273
Authors: 
Shiney Hangloo[1], Anuradha Sourirajan[1], Chand Raina[2], Asha Chaubey[2] and Kamal Dev*[1]
Address: 
[1] Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, Himachal Pradesh, India.
Address: 
[2] Fermentation Technology Division, Indian Institute of Integrative medicine, canal road, Jammu, India.

The aim of this study was to isolate and select lipase producing bacteria from effluent waste of paint industry. Five lipase producing Gram negative bacterial isolates showed promising extracellular lipase activity. Molecular identification on the basis of 16S rDNA identified bacterial isolates as Aeromonas sp. Sh 2 (KT186103.1), Aeromonas sp. Sh 8 (KT229540.1), Aeromonas sp. Sh 12 (KT427918.1), Pseudomonas sp. Sh 13 (KT251204.1), Pseudomonas sp. Sh 17 (KT251205.1).

Keywords: 
Lipase, p-nitrophenyl palmitate, 16S rDNA, paint industry waste, Aeromonas, Pseudomonas

Indirect somatic embryogenesis and evaluation of genetic fidelity of in vitro propagated plants of Bunium persicum (Boiss.) Fedtsch by using DNA markers

Order of Publishing in Issue: 
4
Volume :10
Issue :3
July, 2016
Page No: 
237-247
Authors: 
Rajinder Kaur, Neha Sharma[1]*, Sadiq Majeed and Yogeeta Thakur[2],[3]
Address: 
Department of Biotechnology, Dr. Y.S.Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) 173230
Address: 
[1]Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India.
Address: 
[2]Central Coordinating Unit, Horticulture Mission for Himachal Pradesh at CPRI Shimla.
Address: 
[3]Central Potato Research Institute, Shimla
Email-ID: 
nehabtc@gmail.com

A reproducible and efficient protocol was developed for inducing indirect somatic embryogenesis using callus originated from different explants of Bunium persicum viz. both immature and mature zygotic embryos, fruit pedicel and cotyledons excised from in vitro germinated seedlings. Calli derived from different explants was inoculated on Murashige and Skoog (MS) basal medium of half and full strength supplemented with various concentrations of thiadiazuron, indole butyric acid and with activated charcoal.

Keywords: 
Micropropagation, medicinal plant, regeneration, growth regulators, somatic embryogenesis

Pharmacokinetic analysis of atorvastatin against experimental hepatotoxicity with special reference to CYP3A4 functioning in rats

Order of Publishing in Issue: 
3
Volume :10
Issue :3
July, 2016
Page No: 
229-236
Authors: 
M. Anudeep Reddy, B. Kala Kumar, G. Boobalan*, M. Vijay Kumar, M. Kasi Reddy, C.S.V. Satish Kumar and A. Gopala Reddy
Address: 
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500 030, India.
Email-ID: 
bhupalvets@gmail.com

Aim: To assess the functional status of CYP3A4 substrate in experimental induced hepatotoxicity treated with N-acetyl L-cysteine and Green tea extract for a period of 14 days in rats.

Keywords: 
Acetaminophen, Atorvastatin, CYP3A4, Green Tea, N-Acetyl L-cysteine

Homology Modeling of Endoxylanase from Trichoderma pseudokoningii

Order of Publishing in Issue: 
2
Volume :10
Issue :3
July, 2016
Page No: 
222-228
Authors: 
Bandikari Ramesh, Seelam Naga Sivudu, Katike Umamahesh and Obulam Vijaya Sarathi Reddy*
Address: 
Department of Biochemistry, Sri Venkateswara University, Tirupati - 517 502, India.
Email-ID: 
ovsreddy@yahoo.com

Endoxylanase is involved in the degradation of xylan to xylose. It majorly breaks down the hemicelluloses, which are the major components of the cell wall of the plants. In the present study the 3D modeling of endoxylanase from Trichoderma pseudokoningii (B0FXM0) was performed by comparing modeling approach using endo 1-4 β-xylanase from Trichoderma reesei (PDB ID: 1XYP) as template in modeler7V.7 program server.

Keywords: 
Homology modeling, Modeller, Ramachandran plot, Xylanase

Identification of Secreted Proteins during Protease- Activated Receptor 2 Activation in Gastrointestinal Smooth Muscle Cells

Order of Publishing in Issue: 
1
Volume :10
Issue :3
July, 2016
Page No: 
194-220
Authors: 
Sorratod Bosuwan[1], Sittiruk Roytrakul[2], Karnam S. Murthy[3] and Wimolpak Sriwai[4]*
Address: 
[1] Department of Medical Technology, School of Allied Health Sciences, Thammasat University, PathumThani, 12121, Thailand
Address: 
[2] Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology, PathumThani, 12120, Thailand
Address: 
[3] Department of Physiology, VCU Program in Enteric Neuromuscular Sciences, Virginia Commonwealth University, Richmond, Virginia, United States of America
Address: 
[4] Department of Medical Technology, School of Allied Health Sciences, Thammasat University, PathumThani, 12121, Thailand
Email-ID: 
sriwai@tu.ac.th

Protease-activated receptor 2 is a member of G protein-coupled receptor and expressed in a wide variety of cells. PAR2 is involved in several physiological and pathophysiological processes, including growth and development, inflammation, migration, and apoptosis depending on cell types. However, the role of PAR2 in the context of secretory function of inflammatory proteins in smooth muscle cells remains unknown. The aim of the present study was to determine the secretory proteins involved in inflammatory process upon PAR2 activation in smooth muscle cells.

Keywords: 
Protease-activated receptor 2, PAR2, Proteomics, LC-MS, Inflammation

NEWS ITEM

Order of Publishing in Issue: 
11
Volume :10
Issue :2
April, 2016 - June, 2016
Page No: 
i-vi
Authors: 
Editor
Email-ID: 
krssrao@abap.co.in

NIL

Keywords: 
NIL

Phytochemistry and pharmacology of traditionally used medicinal plant Cordia dichotoma Linn (Boraginaceae)

Order of Publishing in Issue: 
10
Volume :10
Issue :2
April, 2016 - June, 2016
Page No: 
186
Page No: 
193
Authors: 
Md. Azizur Rahman[1]* and Juber Akhtar[2]
Address: 
[1] Bioactive Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh (INDIA)- 226026
Address: 
[2] Department of Pharmaceutics, Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh (INDIA)- 226026.
Email-ID: 
marahman@iul.ac.in

Cordia dichotoma is a tall tree which grows in Sri Lanka, India and other warmer countries. Its medicinal properties are known since long time and it is traditionally used to cure several ailments. Its fruits are used as expectorant, astringent, coolant, emollient, purgative and anthelmintic. Anti-inflammatory, analgesic, hepatoprotective and several other pharmacological activities have also been reported from the plant.

Keywords: 
Cordia dichotoma, Pharmacology, Phytochemistry, Traditional uses

 
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