Antiproliferative and antiangiogenic effects of partially purified Luffa acutangula fruit extracts on human lung adenocarcinoma epithelial cell line (A-549)

Order of Publishing in Issue: 
7
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
396-404
Authors: 
Boreddy Purushotham Reddy, R. Kannaiah Goud, S. Venkata Mohan*, P. N. Sarma
Address: 
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology Hyderabad 500 607, India
Email-ID: 
vmohan_s@yahoo.com

Abstract : The present study was undertaken to evaluate the possibility of Luffa acutangula (cucurbitaceae family) fruit as a potential anticancer agent by examining antiproliferative and antiangiogenic activities. Fruit methanolic extract  showed significant antiproliferative activity (IC-50, 131.63±2.31 ug/ml) on human lung adenocarcinoma epithelial cell line (A-549). The extractwas partially purified by chromatography.

Keywords: 
Vascular endothelial growth factor (VEGF), Matrix metalloproteinases MMP), Extracellular matrix (ECM), Chick chorioallantoic membrane (CAM) model.

Purification and characterization of Human Intestinal alkaline phosphatase and its role in the colonization of Helicobacter pylori in the duodenum

Order of Publishing in Issue: 
6
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
389-395
Authors: 
P. V. G. K. Sarma [1]*, O. Hari Prasad, U. V. Prasad, M. Manohar Reddy, M. Kumar Swamy Reddy [2] and G. Subramanyam [3].
Address: 
[1] Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences; Tirupati, India
Address: 
[2] Department of Pathology, Sri Venkateswara Institute of Medical Sciences; Tirupati, India
Address: 
[3] Department of Cardiology, Director, Sri Venkateswara Institute of Medical Sciences, Tirupati, India
Email-ID: 
pvgksarma@gmail.com

Abstract : Intestinal alkaline phosphatase (IAP) from  normal duodenum was concentrated by 0-35% ammonium sulphate and was fractionated in DEAE cellulose column and thus, partially purified IAP was purified by passing through Sephadex G-75 column in all the steps the purification was monitored through enzyme activity. The purity of the IAP was established on C-18 RPHPLC which gave single peak at a retention time of 15 minutes and SDS-PAGE analysis showed single band with molecular weight of 66 KD.

Keywords: 
H. pylori, Intestinal alkaline phosphatase, Sepsis, Colonisation.

An integrated approach for production of cephamycin C using Streptomyces clavuligerus NT4: Sequential optimization of production medium and effect of amino acids

Order of Publishing in Issue: 
4
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
372-384
Authors: 
Baburao Bussari, Shrikant A. Survase*, Parag S. Saudagar and Rekha S. Singhal
Address: 
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400 019, India

Abstract : The present work reports on an integrated approach of media optimization and amino acid supplementation for production of cephamycin C by submerged culture fermentation of Streptomyces clavuligerus NT4. The fermentation was carried out for 144 h at 28 ± 2 ?C on an orbital shaker rotating at 180 rpm. In the first step, the effects of media constituents such as carbon and nitrogen sources were investigated followed by optimization using response surface methodology (RSM).

Keywords: 
Cephamycin C, Response surface methodology, Amino acids, Submerged fermentation, Streptomyces clavuligerus.

Influence of Environmental Factors on siderophore production by Streptomyces fulvissimus ATCC 27431

Order of Publishing in Issue: 
3
Volume :3
Issue :4
October, 2010 - December, 2010
Page No: 
362-371
Authors: 
M. S. Bendale, B. L. Chaudhari and S. B. Chincholkar*
Address: 
School of Life Sciences, North Maharashtra University, P B 80, Jalgaon 425001, Maharashtra, India

Abstract : Streptomyces fulvissimus ATCC 27431 was studied for siderophore production. Biosynthesis of siderophore was found to be influenced by environmental factors. Out of various media tried, chemically defined low iron medium gave maximum siderophore production (94 % siderophore units). A number of carbon and nitrogen compounds were tested for their effect on growth of Streptomyces fulvissimus and siderophore production.

Keywords: 
Siderophore, Streptomyces fulvissimus, Carbon source, pH.

Inhibitory action of hydroxytyrosol from glucose-induced Insulin deficient and pancreas and liver toxicity in vitro

Order of Publishing in Issue: 
2
Volume :3
Issue :4
October, 2010 - December, 2010
Page No: 
352-361
Authors: 
Khaled Hamden [a]*, Nourreddine allouche [b], Mohamed Damak [b], Serge Carreau [C] and Abdelfattah Elfeki [a]
Address: 
[a] Animal Ecophysiology, Faculty of Sciences, Sfax, Tunisia
Address: 
[b] Chemistry of Natural substances laboratory, Faculty of Sciences, Sfax, Tunisia
Address: 
[C] USC 2006 INRA- EA 2608, Biochemistry-University of Caen, France.

Abstract

Keywords: 
Hydroxytyrosol, Pancreas, Glucose toxicity, Insulin, Antioxidant, Liver slices.

In Utero Low-Protein Diet Alters Vascular Estrogen Receptors,Endothelial Nitric Oxide Synthase and Angiotensin 11 Subtype 1 Receptors in adult male and female rat offspring

Order of Publishing in Issue: 
1
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
341-351
Authors: 
Pandu R. R. Gangula [1], Luckey Reed [2] and Chandrasekhar Yallampalli [2]
Address: 
[1] Department of Obstetrics and Gynecology, Meharry Medical College, Nashville, TN, USA
Address: 
[2] Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas, USA
Email-ID: 
pgangula@mmc.edu
Email-ID: 
hyallam@utmb.edu

Abstract
Background/Aims: We investigated whether: 1) in utero low-protein diet (LPD) induced adult hypertension alters vascular estrogen receptor (ER)-related mechanism in both male and female vascular tissues; and whether 2) flutamide, a specific, non-steroidal competitive antagonist of the androgen receptor, improved this system in female offspring. Methods: Pregnant rats were fed either  with 20% protein (control), or 6% protein (LPD) from day 1 (LPD-1) or day 12 (LPD-2) of

Keywords: 
Low-protein diet, estrogen receptors, nitric oxide, angiotensin receptor, flutamide.

Gibberellic Acid and Cycloheximide Influenced the Growth and Biochemical Constituents of a Medicinally Important Plant - Trachyspermum ammi (l.) Sprague

Order of Publishing in Issue: 
13
Volume :4
Issue :1
January, 2010 - March, 2010
Page No: 
596-603
Authors: 
Haribabu Narra [1]*, Praveen Mamidala [2] and P M Mehta [3]
Address: 
[1] Department of Botany, SPDM College, Shirpur – 425 405, Maharashtra, India
Address: 
[2] Department of Plant Sciences, University of Hyderabad, Hyderabad – 500046, India
Address: 
[3] Department of Biosciences, Sardar Patel University, VV Nagar – 388120, India
Keywords: 
Caraway, GA3, Growth, Cycloheximide, Reducing sugars, Total sugars, Total amino acids, Total Proteins.

Evaluation of in-vitro Cultured Cells of Withania somnifera for Antioxidant Activity

Order of Publishing in Issue: 
12
Volume :4
Issue :1
January, 2010 - March, 2010
Page No: 
589-595
Authors: 
Ashish Baldi*, Wazid Hussain and Yogendra Tailor
Address: 
College of Pharmacy, Dr. Shri R.M.S. Institute of Science & Technology, Bhanpura, Distt.-Mandsaur (M.P.) 458775, India
Email-ID: 
baldiashish@gmail.com
Keywords: 
Antioxidant, Free radical scavenging capacity, Withania somnifera, Withanolides.

Pharmacokinetics and In Vitro and In Vivo Correlation of NNdimethylaminocurcumin (NNDMAC) Loaded Polycaprolactone Microspheres in Rats

Order of Publishing in Issue: 
10
Volume :4
Issue :1
January, 2010 - March, 2010
Page No: 
578-588
Authors: 
Jithan Aukunuru* and Vidyavathi Sankavarapu
Address: 
Vaagdevi College of Pharmacy, Ramnagar, Hanamkonda-506001, India
Email-ID: 
aukunjv@gmail.com

Keywords: 
NNDMAC, microspheres, sutained release, pharmacokinetics, IVIVC

High Yield Expression of Human Recombinant PTH (1-34)

Order of Publishing in Issue: 
10
Volume :4
Issue :1
January, 2010 - March, 2010
Page No: 
568-577
Authors: 
Srinivasa R. Gangireddy [1,2], Radha D. Madhavi [1], Kosana R. Ravikanth [1], Praveen K. Reddy [1], Venkat R. Konda [1], K R S Sambasiva Rao [2]* and Sripad Gunwar [1]
Address: 
[1] Virchow Research Institute, Hyderabad 500055, India
Address: 
[2] Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, India
Email-ID: 
krssrao@yahoo.com

Keywords: 
Factor Xa; Glutathione S transferase; Protein purification, Recombinant human parathyroid hormone.

 
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