Modeled Structure of Trypanothione Synthetase of Leishmania infantum for development of novel therapeutics for leishmaniasis

Order of Publishing in Issue: 
3
Volume :2
Issue :2
July, 2008
Page No: 
390 -395
Authors: 
Bishal Kumar Singh, Nandini Sarkar and Vikash Kumar Dubey *
Address: 
Department of Biotechnology, Indian Institute of Technology Guwahati- 781039, Assam, India
Email-ID: 
vdubey@iitg.ernet.in

Trypanothione synthetase (TryS) is an important enzyme for survival of Leishmania; thus an important target for structure based drugdesign against leishmaniasis. We have constructed three-dimensional structure of the TryS of Leishmania infantum by homologymodeling with acceptable Ramachandran statistics. The modeled structure TryS is compared with human (host) glutathionesynthetase (GS) and Escherichia coli glutathionyspermidine synthase (GspS) involved in similar functions. Remarkable structural

Keywords: 
Structure-Function; Synthetase activity, Homology Modeling

Enhanced artemisinin production by cell cultures of Artemisia annua

Order of Publishing in Issue: 
12
Volume :2
Issue :2
March, 2008
Page No: 
12-128
Authors: 
Ashish Baldi* 1 and V.K. Dixit 2
Address: 
1 Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India
Address: 
2 Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar (M.P.) 470003, India
Email-ID: 
baldiashish@gmail.com

Artemisinin, an endoperoxide containing sesquiterpene lactone from Artemisia annua, hasproven very effective in treating drug resistant cases of malaria and cancer. To counter thepresent low content in leaves and uneconomical chemical synthesis, alternate ways to produceartemisinin have been sought. Inspite of extensive work in this area, artemisinin remainselusive in dedifferentiated and differentiated cultures of A. annua. This work reports the firstsuccessful approach for production of artemisinin by cell cultures of A. annua cultures of Indian

Keywords: 
Artemisinin, Artemisia annua, Hairy roots, Agrobacterium rhizogenes

Studies on Designing, Construction, Cloning and Expression of a novel synthetic antimicrobial peptide

Order of Publishing in Issue: 
11
Volume :2
Issue :2
March, 2008
Page No: 
114-120
Authors: 
P Jawahar Babu, KK Pulicherla, VPB. Rekha1, R Nelson2 and KRS Sambasiva Rao*
Address: 
Center for Biotechnology, Acharya Nagarjuna University, Guntur-522510, India
Address: 
1 Department of Biotechnology, JJ college of Arts and Science, Puddukottai- 622 507, India
Address: 
2 Department of Botany, Government Arts College, Ariyalur - 621713, Tamilnadu, India
Email-ID: 
krssrao@yahoo.com

In the present study a 9 amino acid synthetic anti microbial peptide was designed byusing the bioinformatics tools viz., ProtParam, Hierarchical Neural Network, Anti microbialpeptide database. The designed peptide was back translated to core nucleotide sequence and thegene for the peptide was constructed by over lapping PCR methodology. Synthesized genewas cloned into pRSET-A vector and further the peptide was expressed in E.coli host systems-

Keywords: 
Anti Microbial Peptide, Synthetic Peptides, Cationic peptide.

Ameliorative potential of aqueous extract of Pterocarpus marsupium Roxb bark on diabetes associated metabolic alterations

Order of Publishing in Issue: 
10
Volume :2
Issue :2
March, 2008
Page No: 
107-113
Authors: 
M. Gayathri and K. Kannabiran*
Address: 
School of Biotechnology, Chemical and Biomedical Engineering VIT University, Vellore-632 014, Tamil Nadu, India.
Email-ID: 
kkb_67@yahoo.com

In this study, have the effect of oraladministration of aqueous extract of the bark ofPterocarpus marsupium Roxb (PM) for a periodof 12 weeks on diabetes associated metabolicalterations in normal and streptozotocin (STZ)induced diabetic rats has been studied. Theparameters checked are plasma insulin,glycosylated hemoglobin, total cholesterol,HDL-cholesterol, LDL-cholesterol, triglycerides,alkaline phosphatase (ALP), aspartatetransaminase (AST), alanine transaminase(ALT), ã-glutamyl transferase (ã GT) and creatine

Keywords: 
Pterocarpus marsupium Roxb, hemoglobin sugar, insulin, lipid profile, serum marker enzymes

Butyl paraben induced changes in the biophysical properties of dipalmitoyl phosphatidic acid vesicles

Order of Publishing in Issue: 
9
Volume :2
Issue :2
March, 2008
Page No: 
96-106
Authors: 
Lata Panicker,*

The perturbation in the biophysical properties of multilamellar vesicular (MLV) and unilamellar vesicular (ULV) of dipalmitoylphosphatidic acid (DPPA) by the preservative butyl paraben(BPB) has been studied using DSC and (1H and 31P) NMR. DSC results indicate that the mechanism by which BPB interacts with the DPPA vesicles is similar in MLV and ULV. BPB affected the thermotropic phase transition and the molecular mobility of the DPPA vesicles.

Keywords: 
DPPA, differential scanning calorimetry, NMR, Parabens,

Down regulation of MHC-II expression in serial passages of allogenic bone marrow derived stromal cells for cell based therapy

Order of Publishing in Issue: 
8
Volume :2
Issue :2
March, 2008
Page No: 
89-95
Authors: 
Lakshmi Kiran Chelluri*, Vasantha Adavi, G P V S. Subbaiah, K S. Ratnakar and K. Ravindranath
Address: 
Transplant Biology & Stem Cells, Global Hospitals, Hyderabad-500004, India
Address: 
Correspondence:Lakshmi Kiran Chelluri,Ph.D Head, Transplant Biology & Stem Cell Global Hospitals, Lakdi-ka- Pool, Hyderabad - 500 004, India
Email-ID: 
apparusu@hotmail.com

Mesenchymal stem cells (MSC) show evidence of being potential candidates for allogenic application; however test methods toevaluate their suitability are not yet well established. The study evaluates the possible role of histocompatible markers for routine preclinicalscreening of MSCs for cell based therapies. The results in 5 healthy, prospective, allogenic donor-recipient pairs in co-culture

Keywords: 
Mesenchymal stem cells (MSC), Hypoimmunogenicity, Stro-1, MLR, MSC crossmatching.

Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell Permeable Nucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans

Order of Publishing in Issue: 
7
Volume :2
Issue :2
March, 2008
Page No: 
66-84
Authors: 
Vinc Boyd1, Olivia Maria Cholewa2*, Klearchos K. Papas1
Address: 
1Diabetes Institute for Immunology and Transplantation, University of Minnesota, 425 Harvard, Minneapolis, MN 55455, USA
Address: 
2Molecular Probes, Inc., 29851 Willow Creek Road, Eugene, OR 97402, USA

A review of current literature shows that the combined use of the cell permeable esterasesubstratefluorescein diacetate (FDA) and the cell impermeant nucleic acid stain propidium iodide(PI) to be one of the most common fluorescencebased methods to assess the viability of isolatedislets of Langerhans, and it is currently used for islet product release prior to transplantation inhumans. However, results from this assay do not correlate with islet viability and function or islet

Keywords: 
Fluorescein Diacetate, Propidium Iodide, Viability, Islets of Langerhans.

Role of L-Carnitine and DL- á -Lipoic acid on EEG pattern in aged Rat brain

Order of Publishing in Issue: 
6
Volume :2
Issue :2
March, 2008
Page No: 
57-65
Authors: 
M. Anusuyadevi,1,2 K.S. Jayachandran,3 and C. Panneerselvam,1*
Address: 
1Department of Medical Biochemistry, Dr. ALM. PG.Institute of Basic Medical Sciences, Taramani Campus, University of Madras, Chennai – 600113, India.
Address: 
2Current Address: Department of Physiology and Neurosciences, 173, Ashley Avenue, BSB204, Charleston, SC 29403, United States.
Address: 
3Department of Biochemistry, Guindy Campus, University of Madras, Chennai – 600025, India

Aging is one of major risk factor for neurodegenerative disorders. Understanding the normal and healthy aging is still under progress. The analysis of brain field potentials recordedas electroencephalogram (EEG) has been proven to be one of the sensitive tools to characterize brain aging. In the present study, we examined the EEG pattern of young (4 months) and oldwistar rats (24 months old) for beta and theta wave pattern in frontal and parietal cortical lobesand also the role of Na+/K+ATPase studied in context, that altered electrical activity is coupled

Keywords: 
Aging, EEG, Na+/K+ATPase, carnitine, Lipoic acid, oxidative stress.

Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers

Order of Publishing in Issue: 
5
Volume :2
Issue :2
March, 2008
Page No: 
49-56
Authors: 
B. Rama Rao1, D. Vijay Kumar1, R. Naga Amrutha2, N. Jalaja2, K. Vaidyanath2, A. Maruthi Rao3, Srinath Rao4 Rathnagiri Polavarapu5 and P. B. Kavi Kishor2
Address: 
1Natural Products Laboratory, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 007, India
Address: 
2Department of Genetics, Osmania University, Hyderabad 500 007, India
Address: 
3Department of Botany, Telangana University, Nizamabad 503 002, India
Address: 
4Department of Botany, Gulbarga University, Gulbarga 585 106, India
Address: 
5Genomix Molecular Diagnostics Pvt. Ltd., Balanagar, Hyderabad 500 072, India
Email-ID: 
pbkavi@yahoo.com

Berberine, an isoquinolene alkaloid, together with its related analogs protoberberineand palmatine were detected in cell suspension cultures derived from leaf explants of Tinosporacordifolia. Maximum production of biomass (9.9 g dry cell wt/l) was noticed on day 36 whileberberine was highest (5.5 mg/g dry weight) at day 24. Media pH, plant growth regulators andcarbon sources were optimized for biomass and accumulation of berberine in Linsmaier andSkoog’s medium. Out of 8 cell lines selected, only one line accumulated high berberine (13.9

Keywords: 
Berberine, suspension cultures, Tinospora cordifolia.

Optimization of culture conditions of a novel glucan producing glucansucrase from Leuconostoc dextranicum NRRL B-1146

Order of Publishing in Issue: 
3
Volume :2
Issue :2
March, 2008
Page No: 
40-48
Authors: 
Avishek Majumder and Arun Goyal*
Address: 
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India

The effect of nutrients on the production of glucansucrase from Leuconostoc dextranicumNRRL B-1146 was studied using one variable at a time approach. The culture conditions forglucansucrase production were also optimized. The maximum enzyme production was obtainedat an optimum temperature of 28°C and under static culture condition. The increase in sucroseconcentration from 2% (control) to 4% in the culture medium gave an increase ofglucansucrase activity by 25%. Yeast extract did not affect the enzyme production. However, other

Keywords: 
glucansucrase; Leuconostoc dextranicum; glucan

 
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