In-silico Prediction of T and B cell Epitopes in the Evolutionary Conserved Pathway of Glycolysis for Human Pathogens: Coccidiodes immitis, Histoplasma capsulatum and Pneumocystis carnii

Order of Publishing in Issue: 
2
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
242-252
Authors: 
Neelabh and Karuna Singh*
Address: 
Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi-221005, India

Fungal diseases are amongst the emerging diseases to which humans are most susceptible pertaining to the present day life style. New drugs are being made but at a slow pace, not matching the resistance developing capacity of the fungal pathogens. Therefore, it is important to choose new drug targets peculiar to fungi but absent in humans.A common practice till date has been to use the virulence proteins in order to devise medicines against micro-organisms.

Keywords: 
In-silico, Epitope, Motif, Glycolysis, Fungi.

Optimization of Process Parameters for Bioactive Metabolite Production by Nocardiopsis trehalosi VSM-13 Using Response Surface Methodology and Unstructured Kinetic Modelling

Order of Publishing in Issue: 
1
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
223-241
Authors: 
Ushakiranmayi Managamuri[1], Muvva Vijayalakshmi[1]*, V.S Rama Krishna Ganduri [2, 3], Satish Babu R[4] and Sudhakar Poda[3]
Address: 
[1]Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India
Address: 
[2]Department of Biotechnology, K L University, Vaddeswaram, Guntur, Andhra Pradesh, India
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-52510, Andhra Pradesh, India.
Address: 
[4]Dept of Biotechnology, National Institute of Technology, Warangal, Telangana, India.
Email-ID: 
profmvl08@gmail.com

Nocardiopsis trehalosi VSM 13, an actinobacterium isolated from marine environment was tested for the optimum culture conditions in shake-flask fermentations using one-factor-at-atime method. Response Surface Methodology (RSM) based Central composite Design was used to design the experiments, build the model and determine the optimum conditions for the desirable responses.

Keywords: 
Nocardiopsis trehalosi, Bioactive metabolites, Regression analysis, Statistical optimization.

News Item

Order of Publishing in Issue: 
12
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
i-iv
Authors: 
Editor
Email-ID: 
editor@abap.co.in
Keywords: 
News item

Biological Actions of PPAR- Gamma in Health and Disease

Order of Publishing in Issue: 
11
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
206-222
Authors: 
Seema Thakur* and Neha Srivastava
Address: 
Faculty of Pharmaceutical Sciences, PCTE Group of Institutes, Ludhiana, Punjab, India

Peroxisome proliferator-activated receptors (PPARs) are ligand activated transcription factors that modulate target gene expression in response to endogenous and exogenous ligands. Peroxisome proliferator-activated receptors are expressed in many tissues, including adipocytes, hepatocytes, muscles and endothelial cells. The PPARs, a family of nuclear receptors (NRs), are a set of three receptor sub-types encoded by distinct genes.

Keywords: 
PPAR-gamma, Inflammation, Adipose tissue, Insulin sensitivity, Cancer.

The Vaginal Microbiota in Women Health and Disease: Current Understanding and Future Perspectives - A Review

Order of Publishing in Issue: 
10
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
190-205
Authors: 
Krishna SBN*, Stacey L Wilson and Jamila K Adam
Address: 
Department of Biomedical & Clinical Technology, M L Sultan Campus, Durban-4000, Republic of South Africa.
Email-ID: 
sureshk@dut.ac.za

Vaginal Microbiota varies within individuals and between human populations. Colonization by Lactobacillus in the female genital tract is thought to be critical for maintaining genital health. Nevertheless, little is known about how genital microbiota influence host immune function and modulate disease susceptibility. This article discusses the advancement of new strategies for disease diagnosis and personalized treatments to promote health and improve the quality of women’s lives.

Keywords: 
Microbiome, Health, Lactobacilli, Genital Tract, Quality of Life, Immune regulation, Reproductive age.

CRISPR: Genome -Editing and Beyond

Order of Publishing in Issue: 
9
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
181-89
Authors: 
Sumiyah Rasool[*], Tufail Hussain[@], Asima Zehra[#], Shafat Khan[$] and Shafqat Khan[*]
Address: 
[*]Department of Microbiology, [#]Department of Public Health, [@]Department of Medicine, [$]Department of Horticultural Crop processing, SKUAST-K, Srinagar. J&K, 190001, India
Email-ID: 
szari720@gmail.com

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system has been seized upon with a fervor enjoyed previously by small interfering RNA (siRNA) and short hairpin RNA (shRNA) technologies and has enormous potential for high-throughput functional genomics studies. Editing via (CRISPR)–CRISPR associated (Cas) constitutes a next-generation method for programmable and high through put functional genomics.

Keywords: 
CRISPR, Bacterial immunity, Gene editing.

Statistical Optimization for Tannase Production by Mucor circinelloides Isolate F6-3-12 under Submerged and Solid State Fermentation

Order of Publishing in Issue: 
8
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
167-180
Authors: 
El-Refai, H.A.*, Mohammed A. Abdel-Naby, Hanan Mostafa, M.A., Magdy A. Amin[1] and Hebat-Allah A. Salem
Address: 
Department of Chemistry of Natural and Microbial Products, National Research Center, Cairo, Egypt
Address: 
[1]Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Egypt
Email-ID: 
dr.heba_ar@yahoo.com

Mucor circinelloides isolate F6-3-12 was selected out of three fungal isolates that can utilize tannic acid as substrate. The fungus was identified by phenotypic analysis and 18S rRNA gene sequencing. Extracellular tannase was produced by solid-state fermentation (SSF) and submerged fermentation (SmF) from plants known for their high tannin content to be used as sole carbon source and supporting solid substrate namely pomegranate rind, sumac leaves and green tea leaves powder , pomegranate rind was selected for maximum tannase production under SSF.

Keywords: 
Mucor circinelloides, Tannase, 18S rRNA gene sequencing, Sequential statistical strategy, Solid-state fermentation.

Algorithm to Simulate a Chemically Induced DNA Logic Gate and Boolean Circuit

Order of Publishing in Issue: 
7
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
160-166
Authors: 
Kuntala Boruah*, Rashmi Deka and Jiten Ch Dutta
Address: 
Department of Electronics and Communication Engg. Tezpur University, Tezpur - 784028, Assam, India
Email-ID: 
kuntala17@gmail.com

A simple proof-of-principle type of simulation of chemically implemented logic gate is proposed in this paper using the structural switching of single stranded DNA strand from i-motif to hairpin and vice versa which is triggered by regulating H+ and copper (II) ions. The advantage of such chemically induced gates lies in its fast response time, controllability and reusability feature. Later on this simulation process is expressed in the form of algorithm to evaluate AND-OR Boolean circuit.

Keywords: 
DNA, AND gate, OR gate, Boolean Circuit.

Purification Strategies for Microbial Pectinases

Order of Publishing in Issue: 
6
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
144-159
Authors: 
E. Venkatanagaraju[1]* and G. Divakar[2]
Address: 
[1]Therapeutic Enzymes (Division), Department of Life Sciences, Garden City College of Science and Management Studies, 16th KM, Old Madras Road, Bangalore-560049, Karnataka, India.
Address: 
[2]Department of Pharmaceutical Biotechnology and Microbiology, Acharya & BM Reddy College of Pharmacy, Soldevanahalli, Hesaraghatta, Bangalore-560107, Karnataka, India.

Microbial pectinases currently occupy a place of prominence among biocatalysts owing to their capability to catalyze a diversity of reactions in aqueous and non-aqueous media. Specific action of these enzymes has initiated remarkable attention among industrialists and researchers. Pectinases from a large number of fungal and a few bacterial sources have been purified to homogeneity.

Keywords: 
Bacterial, fungal, Pectinases, Purification.

Screening, optimization and production of a novel beta- cyclodextrinase by Bacillus flexus MSBC 2

Order of Publishing in Issue: 
5
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
136-143
Authors: 
Shwetha V[1], Sunil S More[2]* and Uday M[3]
Address: 
[1]Department of Biochemistry, Center for Post Graduate Studies,Jain University, Bangalore- 560011, India
Address: 
[2]School of Basic and Applied Sciences, Dayananda Sagar University, K S Layout, Bangalore-560078, India
Address: 
[3]Department of Biotechnology, BVB College of Engineering and Technology, Vidyanagar, Hubli-590031, India
Email-ID: 
sunilsmorey@gmail.com

CGTase (Cyclodextrin Glycosyl Transferase), is an important starch modifying enzyme catalyzing conversion of starch and related polysaccharide into cyclodextrins (CDs), which have important applications in numerous industries. In the present study, a potent CGTase producing bacteria was isolated from corn field soil. The bacteria was identified by microscopic and biochemical tests along with 16s rRNA to be as Bacillus flexus MSBC 2. From plate assay it was seen that only β- CD was produced.

Keywords: 
CGTase, Bacillus flexus MSBC 2, β- cyclodextrin.

 
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