Sonic Stimulation can affect Production of Quorum Sensing Regulated Pigment in Serratia marcescens and Pseudomonas aeruginosa

Order of Publishing in Issue: 
3
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
121-128
Authors: 
Vijay Kothari*, Pooja Patel, Chinmayi Joshi, Brijesh Mishra, Shashikant Dubey and Milan Mehta
Address: 
Institute of Science, Nirma University, Ahmedabad, India.

Effect of nine different mono-frequency sound stimuli on two gram-negative bacteria (Pseudomonas aeruginosa and Serratia marcescens) was investigated. Frequency of the test sound ranged from 100 Hz – 2,000 Hz. Both the test bacteria responded differently to sonic stimulation. Sound corresponding to 600 HZ caused a notable reduction in quorum sensing (QS) regulated production of the pigment pyoverdine by P. aeruginosa. 400 Hz sound affected prodigiosin production by S. marcescens the most.

Keywords: 
Sound, Quorum sensing, Sonic stimulation, Prodigiosin, Pyoverdine.

Comparative Microbiological and Physico-Chemical Properties of Commercially available Baker’s Yeast and Fruit Juice Isolate

Order of Publishing in Issue: 
2
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
113-120
Authors: 
Hiranmay Malik, Priya Katyal* and Param Pal Sahota
Address: 
Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana-141004, Punjab, India.
Email-ID: 
drpkatyal@pau.edu

Seven commercially available Baker’s yeasts formulations were procured from local market and stored under the conditions mentioned by the manufacturer. Microbiological analysis of the product was done using five different selective media viz. Rose Bengal chloramphenicol agar for moulds, Baired Staph agar for Staphylococcus aureus, McKonkey agar for faecal coliforms, B. cereus selective agar for Bacillus cereus and Salmonella-Shigella agar for Salmonella.

Keywords: 
Baker’s yeast, Microbial contaminants, Invertase activity, Trehalose, Fermentation.

Multiple Parameter Optimization for Maximization of Exopolysaccharide Production from Lactobacillus paraplantarum KM1 by Response Surface Methodology

Order of Publishing in Issue: 
1
Volume :11
Issue :2
April, 2017 - June, 2017
Page No: 
103-112
Authors: 
Kanika Sharma, Nivedita Sharma* and Ranjana Sharma
Address: 
Microbiology Research Laboratory, Department of Basic Sciences, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan-173230, HP, India

Statistical experimental design was applied to optimize the medium for exopolysaccharides (EPSs) production from Lactobacillus paraplantarum KM1. A design comprising of incubation time, temperature, pH, carbon concentration (lactose) and nitrogen concentration (ammonium sulfate). A quadratic model was found to fit the EPS production. The optimum values of tested variables for EPS production were 25% w/ v lactose, 35æ%C, pH 6.5 and 32 h for cultivation time. A great variation in EPS production was observed that ranged from 0 to 34.6 mg/ml.

Keywords: 
Exopolysaccharides, Lactobacillus paraplantarum, RSM.

Mucorimietiana sp. nov.

Order of Publishing in Issue: 
11
Volume :11
Issue :1
January, 2017
Page No: 
99-102
Authors: 
A. Swathi Sri and A. Subrahmanyam[1]*
Address: 
Department of Biotechnology, Krishna University, Machilipatnam, AP, India
Address: 
[1] 1Department Biotechnology, Meerut Institute of Engineering & Technology (MIET), NH-58 Bypass crossing, Meerut -250005, U.P., India

A thermophilic, heterothallic species of Mucor - M. imietiana A. Swathi Sri.& A.Subrahm.sp. nov.isolated from garden soil from MIET campus is described and illustrated. It grows well at 500 C on synthetic mucor agar and potato-dextrose agar from pH 3.0-9.0 with an optimum pH at 7.0. The specific epithet refers to its habitat.
Key words: Mucorimietiana sp. nov., Thermophilic, Heterothallic, Garden soil MIET Meerut

Keywords: 
Mucorimietiana sp. nov., Thermophilic, Heterothallic, Garden soil MIET Meerut

News Item

Order of Publishing in Issue: 
11
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
i - v
Authors: 
Editor
Email-ID: 
editor@abap.co.in
Keywords: 
News item

Molecular Cloning of Partial Phytase Gene from Bacillus subtilis ATCC 6633 (GenBank: KT385665.1).

Order of Publishing in Issue: 
10
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
91-98
Authors: 
Chaitanya Mogal*, Diwakar Singh, Amit Mehta and Taslim Ahmad.
Address: 
Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari- 396450. Gujarat, India.

Phytase (myo-inositolhexakisphosphate phosphohydrolase) catalyzes the hydrolysis of myo-inositol hexakisphosphate (phytic acid) to inorganic monophosphate and lower myo-inositol phosphates, and in some cases to free myo-inositol. Bacillus species are known to produce a thermostable phytase. The Bacillus subtilis strain ATCC 6633 was chosen for cloning of phy gene. Primers were designed for phy gene amplification using the phy gene sequence of B. subtilis (AF298179).

Keywords: 
Phytase, PCR, phy gene, Molecular Cloning.

Green Synthesis of Silver Nanoparticles using two Apocyanaceae plants and Screening for their Catalytic activity

Order of Publishing in Issue: 
9
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
84-90
Authors: 
Bodaiah Bonigala[1], Usha Kiramayi Mangamuri[2], Anuhya G[1], Yamini Saraswathi Y[1], K.R.S.Sambasiva Rao[1] and Sudhakar Poda[1]*
Address: 
[1]Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, India
Address: 
[2]Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India

The catalytic activity of biosynthesized silver nanoparticles (AgNPs) gained an importance in removal of synthetic dyes from waste water released form textile industries. In this study aqueous leaf extract of Cascabela thevetia and Wrightia tomentosa were used to synthesize silver nanoparticles. Further the catalytic activity of newly synthesized particles in reducing 4- Nitrophenol, Methylene blue, Methyl orange and Methyl red using NaBH4 was screened.

Keywords: 
Green synthesis, AgNPs, 4- Nitrophenol, Synthetic dyes, Catalytic activity

Protective Effects of Zinc and Vitamin-E for Arsenic Induced Mitochondrial Oxidative Damage in Rat Brain

Order of Publishing in Issue: 
8
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
67-83
Authors: 
M. Ram Kumar and G. Rajarami Reddy
Address: 
Department of Zoology, Sri Venkateswara University, Tirupati Andhra Pradesh, 517 502, India.

This study was planned to determine the influence of age on arsenic (As; 10mg/kg body weight given through oral gavage) induced mitochondrial oxidative stress in three different age groups of rats; young (postnatal day [PND] 21), adult (3 months) and aged (18 months) at seven days post-acute exposure. Further, we also evaluated the therapeutic efficacy of essential metal Zinc (Zn; 0.02% through drinking water) and an antioxidant, α-Tocopherol (Vit-E; 125mg/kg body weight through oral gavage) against As-induced neurotoxicity.

Keywords: 
Age, Arsenic, Zinc, Vitamin-E, Oxidative stress, Brain regions

Detection and Production of Gallic acid from Novel Fungal strain- Penicillium crustosum AN3 KJ820682

Order of Publishing in Issue: 
7
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
60-66
Authors: 
Nisha Thakur* and A. K. Nath
Address: 
Department of Biotechnology, Dr Y S Parmar University of Horticulture and Forestry, Solan, 173230, India

Phenolic compound 3,4,5-trihydroxy benzoic acid commonly known as gallic acid, an important multipurpose ingredient harboured from an ascomycetes fungus which was previously isolated from an apple orchard soil and identified as Penicillium crustosum AN3(Gene bank accession no. KJ820682). The product gallic acid was obtained during fungal fermentation studies.

Keywords: 
Gallic acid, Penicillium crustosum AN3, Production optimization, Analytical techniques

Screening and Profiling of Sandal Seeds collected from Provenances of Karnataka

Order of Publishing in Issue: 
6
Volume :11
Issue :1
January, 2017 - March, 2017
Page No: 
53-59
Authors: 
Vasundhara, M[1]*, Nuthan D[2] and G.G.E, Rao[3] and Priyanka R[4]
Address: 
[1,4] Medicinal and Aromatic Section, Department of Horticulture, UAS(B), GKVK, Bangalore
Address: 
[3] Department of Agronomy, College of Sericulture, UAS (B), Chintamani – 563 125
Address: 
[2] University of Agricultural Sciences, Bangalore, India

Sandalwood trees are the only source of precious natural sandal oil obtained from the heartwood. The tree develops heartwood over a period of twenty years. However, flowering and seed set are observed within two years of planting. Seeds serve as a potential source of an important therapeutic compound and therefore ensures steady income to the growers. Indian Sandalwood and Australian sandalwood are the source of a unique fatty acid called Ximenynic or Santalbic acid. Sandal grows in abundance in many states of South India.

Keywords: 
Santalum album, Ximenynic acid, Gas chromatography, Provenances.

 
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