Identification of Thermophilic Flavobacterium and Anoxybacillus in Unexplored Tatapani Hot spring of Kishtwar District of Jammu and Kashmir: A North Western Himalayan State

Order of Publishing in Issue: 
4
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
245-256
Authors: 
Sonum Sharma[1], Parul Sharma[1], Anuradha Sourirajan[1] David J. Baumler[c,d,e] and Kamal Dev[1c*]
Address: 
[1]Faculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No.9, Head Post Office, Solan (H.P.)-173212.
Address: 
[c]Department of Food Science and Nutrition, [d]Microbial and Plant Genomic Institute, and [e]Biotechnology Institute, University of Minnesota-Twin Cities St. Paul, Minnesota, USA

Two industrially important thermophilic bacteria were isolated from Tatapani hot spring located in Kishtwar district of Jammu and Kashmir, India and were named as KSI and KSII. Water sample from Tatapani hot spring was collected for screening of industrially important thermophillic bacteria. Only two thermophilic bacteria (KSI and KSII) were isolated from Tatapani hot spring water sample. The strict thermophilic nature of both the bacterial isolates (KSI and KSII) was tested by growing them at different temperatures ranging from 40°C-70°C.

Keywords: 
Kishtwar, Himalayas, Flavobacterium thermophilum, Anoxybacillus.

Molecular Characterization and optimization of Bioactive Compounds Production of three Actinomycetes spp Isolated from Waste Dump Soil from Western Uganda

Order of Publishing in Issue: 
3
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
230-244
Authors: 
Adamu Almustapha Aliero[1,2], Abubakar Sunusi Adam[1], Ibrahim Ntulume[1], Ahmad Ibrahim Bagudo[2], Aminu Ado B/Kudu[3], Miruka Conrad Ondieki[4], Sanusi Ahmad Jega[4], John Odda[3], Matilda Angela Okech[5]
Address: 
[1]Department of Microbiology and Immunology, Kampala International University Western campus, Uganda
Address: 
[2]Department Biological Sciences Kebbi State University of Science and Technology, Aliero, Nigeria
Address: 
[3]Department of Pharmacology, Kampala International University-Western Campus, Uganda
Address: 
[4]Department of Biochemistry, Kampala International University-Western Campus, Uganda
Address: 
[5]College of Health, Medicine and Life Sciences, Department of Microbiology, St. Augustine International University, Uganda

The study was designed to investigate the effect of growth culture conditions, namely: Media composition, incubation period, temperature and pH on the production of antimicrobial compounds from three selected actinomycetes isolated from waste dump soil in Western Uganda. Molecular characterizations of these selected isolates were also carried out. Optimization processes were assessed using shake-flask cultures on eight different media, Portion of filtered fermentation broths were assayed using agar well diffusion method.

Keywords: 
Optimization, Actinomycetes spp, Bioactive Compound, Molecular characterization, Waste dump soil, Western Uganda.

Development of Monoclonal Antibodies Against Cry1Ac/ Ab Protein for Designing of Sandwich ELISA to Detect BT Toxin from Cotton Seeds and Leaves

Order of Publishing in Issue: 
2
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
218-229
Authors: 
Seetharavamma Gnagadasu[1&2], Jayant K Bhanushali[1], Usha Kiranmayi Mangamuri[3], K. R. S. Sambasiva Rao[1], Sudhakar Poda[1*]
Address: 
[1]Amar Immunodiagnostics Pvt, Ltd, Plot No 242/1, Road No 18, Jubilee Hills, Hyderabad-500033, Telangana, India
Address: 
[2]Department of Botany and Microbiology and Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India

The design of the study is to develop monoclonal antibodies against Cry1Ac/Ab protein for designing os sandwich ELISA (hybridoma technology). Hybridoma technology was invented by Cesar Milstein and Georges J.F Kohler in the year 1975 and is an unique method used to produce identical antibodies in maximum quantities. Monoclonal antibodies were developed by immunization of Balb/C mice with Cry1Ac/Ab Protein. Titer values of mice tail bleeds were checked and the best mice with higher titer value was used for fusion.

Keywords: 
Bacillus thuringiensis, Hybridoma Technology, Cry1Ac/Ab, HAT medium, Monoclonal antibodies, Iso-typing, genetically modified crops and Immunization.

Chart Review Study of Subjects Administered Amnionic Membrane for Treatment of Joint Pain

Order of Publishing in Issue: 
1
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
212-217
Authors: 
Ruben Berrocal Timmons[1]*, Neal E. Fearnot[2] and Michael E. Leckrone[2]
Address: 
[1]Interventional Rehabilitation Center, Pensacola, Florida, USA
Address: 
[2]Cook Advanced Technologies, West Lafayette, Indiana, USA
Email-ID: 
rbtmd1@gmail.com

Context: Treatment of joint pain with injection of amnionic membrane has not been adequately studied. Objectives: Determine if patients who received cryopreserved particulate amnionic membrane (CPAM) injected into painful back and knee joints experience less back or knee pain, improve physical ability, and use less opiates and NSAIDs over a 12 week time period.

Keywords: 
Amnion, Amnionic membrane, Joint pain, Knee pain, Back pain.

News Item

Order of Publishing in Issue: 
11
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
i - vi
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Items

Keywords: 
News item

From Natural products to therapeutically important antifungals

Order of Publishing in Issue: 
10
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
206-212
Authors: 
Neelabh and Karuna Singh
Address: 
Department of Zoology (MMV), Banaras Hindu University Varanasi -221005, India

Although, there has been a significant progress in the field of pharmaceuticals and drug designing in the recent past, yet there is a vacuum in the development of broad spectrum antifungal drugs. Those drugs that are at our disposal are not free from side effects having several risks and associated toxicities on prolonged usage. Therefore, there has been an ever escalating demand of devising new drugs that are preferably natural and free from side effects.

Keywords: 
Natural, Antifungal, Diseases, Chitin synthase inhibitors.

Targeting Cancer cell metabolism via Target of Rapamycin

Order of Publishing in Issue: 
9
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
196-205
Authors: 
Ankita Awasthi[1], Vikrant Nain[1], Himanshi Singh[1] , Pavan Kumar[1], Rekha Puria[1]*
Address: 
[1]School of Biotechnology, Gautam Buddha University, Greater NOIDA, Gautam Budh Nagar-201312, India
Email-ID: 
rpuria@gbu.ac.in

Cancer cells acquire many metabolic rearrangements to provide energy and macromolecules required for continuous growth and proliferation. Warburg suggested that cancerous cells rely only on glycolysis for energy and biosynthesis of macromolecules. It’s still unexplainable that how this metabolic switch allows predominance of cancer cell in the hypoxic and metabolically highly active conditions around the cancerous cells.

Keywords: 
Nil

Molecular Taxonomy of Associated Microbes From Sea Slug Kalinga Ornata and its bioactivity

Order of Publishing in Issue: 
8
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
187-195
Authors: 
M. Mohanraj[1], N. Sri Kumaran[1,2]* and S. Bragadeeeswaran[1]
Address: 
[1]Faculty of Marine Science, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai – 608 502, Tamil Nadu, India
Address: 
[2]Department of Marine Biotechnology, AMET University, Kanathur, Chennai 603112, Tamil Nadu, India
Email-ID: 
s.kumaran08@gmail.com

This study was carried to investigate the molecular taxonomy of associated microbes from sea slug Kalinga ornata and its bioactivity form Indian coastal waters. In this study, totally 27 associated microbes isolated from sea slug K. ornate. Morphologically 5 different isolates were selected for sequencing and submitted to NCBI. Further these 5 sequences were analysed for the phylogenetic relationship and percentage of the nucleotide contents with few isolates sequences extracted via FASTA format from NCBI.

Keywords: 
Sea slug, Molecular taxonomy, Sequencing, Antagonistic, Heamolytic.

System Modeling of AkT using Linear and Robust Regression Analysis

Order of Publishing in Issue: 
7
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
177-186
Authors: 
Shruti Jain
Address: 
Department of Electronics and Communication Engineering, Jaypee University of Information Technology, Himachal Pradesh

This paper presents the review of different types of modeling like deterministic and probabilistic. Main stress of this paper is on Probabilistic modeling. Probabilistic modeling is further divided into three types : predictive, regression and correlation modeling. The analysis was done using three different input proteins and four different output proteins. A model was made using different analysis techniques which presents the r2 values for one of the marker protein called AkT.

Keywords: 
AkT, Linear Regression Analysis, Robust Regression Analysis, Partial Least Square.

In-Vivo Evaluation of Rifampicin Loaded Nanospheres: Biodistribution and Mycobacterium Screening Studies

Order of Publishing in Issue: 
6
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
169-176
Authors: 
Vishnu Vardhan Reddy Beeram[1], Krupanidhi S[1]*& Venkata Nadh R[2]
Address: 
[1]Department of Biotechnology, Vignan’s University, Vadlamudi, Guntur-522213, India.
Address: 
[2]GITAM University – Bengaluru Campus, Karnataka- 561 203, India.

Rifampicin PLGA nanospheres are formulated with a specific goal in order to decrease the dose, adverse effects and to enhance targeted drug delivery. Rifampicin nanospheres were prepared and evaluated by emulsion solvent evaporation method. In vivo bio distribution studies reveal that there was a long term accumulation of rifampicin nanospheres in the lungs over other organs. The increase in Cmax values confirmed that inhalable PLGA nanospheres are suitable for targeting and providing sustained release of antitubercular drugs to lungs.

Keywords: 
Rifampicin Nanospheres, M. tuberculosis, In vivo Bio distribution studies.

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login